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Detection of nucleic acid hybridization by fluorescence polarization

  • US 20050009174A1
  • Filed: 06/30/2004
  • Published: 01/13/2005
  • Est. Priority Date: 05/12/2000
  • Status: Abandoned Application
First Claim
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1. A system comprising:

  • a container comprising a duplexed nucleic acid disposed in the container, wherein at least one strand of the nucleic acid duplex comprises a neutral or positively charged fluorescent label;

    a polarized light source positioned to shine plane polarized light through a portion of the container, thereby exciting the fluorescent label during operation of the system; and

    , a detector that detects resultant polarization of light emitted by the fluorescent label.

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