Methods and apparatuses for analyzing polynucleotide sequences
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Accused Products
Abstract
Methods for high speed, high throughput analysis of polynucleotide sequences, and apparatuses with which to carry out the methods are provided in the invention.
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Citations
74 Claims
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1-54. -54. (Cancelled)
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55. A method of deriving nucleotide sequence information from individual polynucleotide molecules, the method comprising the steps of:
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locating the respective positions of a plurality of target nucleic acid template molecules in a field of immobilized polynucleotides;
exposing said field to a polymerase and at least one nucleotide comprising a detectable label under conditions that allow said nucleotide to be incorporated in a primer hybridized to said template molecule if said nucleotide is complementary to a nucleotide in said template immediately 5′
of said primer;
determining the position or positions of optical signal emanating from label attached to a nucleotide that has been incorporated in said primer; and
recording the identity and position of an incorporated nucleotide based upon said optical signal. - View Dependent Claims (56, 57, 58, 59, 60, 61, 62, 63, 64)
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65. A method for identifying one or more nucleotides in a nucleic acid target molecule, the method comprising the steps of:
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(a) obtaining an array of support-bound target nucleic acids in which each of said support-bound nucleic acids is individually optically resolvable;
(b) exposing said array to a polymerase, a primer capable of hybridizing to at least some of said support-bound nucleic acids and at least one nucleotide comprising a detectable label under conditions that allow said nucleotide to be incorporated into said primer if said nucleotide is complementary to a nucleotide in said target immediately 5′
to said primer;
(c) washing said array in order to remove unincorporated nucleotides;
(d) determining the presence and position of label attached to an incorporated nucleotide;
(e) neutralizing any label observed in said determining step;
(f) repeating steps a, b, c, and d;
(g) identifying label present at any position in which presence of label has previously been determined in said determining step;
(h) repeating steps a, b, c, d, and e; and
(i) identifying a sequencing of nucleotides in said target nucleic acid based upon the sequence of labels detected at any given position in said array. - View Dependent Claims (66, 67, 68, 71, 72, 73, 74)
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- 69. The method of 68, wherein said inhibition is reversible.
Specification