Liquid delivery apparatus and method
First Claim
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1. A method for delivering a spot of liquid onto a substrate comprising:
- (a) bringing a hollow capillary into contact with the liquid;
(b) locating the capillary above a selected position on the substrate;
(c) delivering a partial drop of liquid from the capillary onto the substrate by relative movement of the capillary and substrate;
(d) retraction of the capillary and substrate relative to each other; and
(e) repeating steps (c) and (d) one or more times.
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Abstract
Liquid delivery apparatus and methods, in particular for the production of high-density arrays, wherein a partial drop liquid is deposited from a capillary (1) onto a substrate (12), the capillary (1) and substrate (12) being capable of x, y and z movements relative to one another under the control of a translation mechanism.
28 Citations
23 Claims
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1. A method for delivering a spot of liquid onto a substrate comprising:
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(a) bringing a hollow capillary into contact with the liquid;
(b) locating the capillary above a selected position on the substrate;
(c) delivering a partial drop of liquid from the capillary onto the substrate by relative movement of the capillary and substrate;
(d) retraction of the capillary and substrate relative to each other; and
(e) repeating steps (c) and (d) one or more times. - View Dependent Claims (2, 3, 4, 5, 6, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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7. A liquid delivery apparatus comprising:
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(a) at least one hollow capillary contactable with the liquid;
(b) a housing to retain said capillary in a desired orientation;
(c) one or more thermal, mechanical or electrical second liquid delivery devices;
(d) a housing to retain said second liquid delivery device in a desired orientation;
(d) a substrate holder; and
(e) a translation mechanism capable of performing x, y and z movements, said mechanism being capable of moving said capillary, second liquid delivery device and substrate holder relative to one another. - View Dependent Claims (8, 9)
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23. A method for producing an array of peptides for mass spectrometric analysis on a solid substrate comprising:
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(a) bringing a hollow capillary into contact with a liquid sample containing polypeptides;
(b) locating the capillary above a selected position on the substrate;
(c) delivering a partial drop of liquid from the capillary onto the substrate by relative movement of the capillary and substrate;
(d) retraction of the capillary and substrate relative to each other (e) repeating steps (c) and (d) one or more times;
(f) relocating the capillary above a different selected position on the substrate and performing steps (c) to (e);
(g) repeating step (f) one or more times; and
(h) applying a matrix solution to the spots of liquid on the substrate before the liquid has completely evaporated.
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Specification