Virtual well plate system

• US 20050019224A1
• Filed: 06/14/2004
• Published: 01/27/2005
• Est. Priority Date: 06/16/2003
• Status: Abandoned Application

• Current Assignee: Schering Corporation (Merck & Co., Inc.)
• Original Assignee: Schering Corporation (Merck & Co., Inc.)
• Inventors: Pechter, David S., Varga, Edward G.
• Application Number:
  US10/867,586
• Publication Number:
  US 20050019224A1
• Time in Patent Office: Days
• US Class Current: 506/13
• CPC Class Codes: B01F 25/14 Mixing drops, droplets or bodies of liquid which flow together or contact each other micromixers using where the materials to be mixed are in the form of droplets B01F33/302
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01F 33/3021 the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
• CPC Class Codes: B01F 33/30351 using hydrophilic/hydrophobic surfaces
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00328 Movement by linear translation
• CPC Class Codes: B01J 2219/00364 Pipettes
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00691 using robots
• CPC Class Codes: B01L 2300/047 Additional chamber, reservoir
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: B01L 3/5088 confining liquids at a location by surface tension, e.g. virtual wells on plates, wires B01L3/50857 takes precedence
• CPC Class Codes: C40B 60/14 for creating libraries

Abstract

A virtual well plate system includes a base including a base plate having an upper surface with a hydrophobic region which defines hydrophilic domains, each hydrophilic domain adapted to hold a droplet of liquid therein; a movable lid including a lid plate having a lower surface with a hydrophobic region which defines hydrophilic domains, each hydrophilic domain adapted to hold a liquid droplet in a hanging manner; and a resistance arrangement mounted to the base and/or lid and which maintains the base and lid in an assembled condition such that the base plate and lid plate are maintained at a sufficient distance to prevent formation of virtual wells by the droplets, and which permits movement of the lid toward the base upon application of an external force sufficient to overcome a resistance of the resistance arrangement, to form virtual wells by combining the droplets.

Claims

1. A virtual well plate system comprising:

a base including a base plate having an upper surface with a hydrophobic region which defines a plurality of hydrophilic domains on the upper surface of the base plate, each hydrophilic domain adapted to hold a droplet of liquid therein;

a movable lid including a lid plate having a lower surface with a hydrophobic region which defines a plurality of hydrophilic domains on the lower surface of the lid plate, each hydrophilic domain of the lid plate adapted to hold a droplet of liquid therein in a hanging manner; and

a resistance arrangement mounted to at least one of the base and the lid which maintains the base and lid in an assembled condition such that the base plate and lid plate are maintained at a sufficient distance to prevent formation of virtual wells by the droplets thereon, and which permits movement of the lid toward the base upon application of an external force sufficient to overcome a resistance of the resistance arrangement in order to form the virtual wells by a combination of the droplets on the base plate and the lid plate

2. A virtual well plate system according to claim 1, wherein the resistance arrangement includes at least one spring which supports the movable lid above the base.

3. A virtual well plate system according to claim 2, further comprising a stationary lid mounted to said base, and the at least one spring is connected between the stationary lid and the movable lid for supporting the movable lid above the base.

4. A virtual well plate system according to claim 3, wherein the at least one spring includes a plurality of springs selected from the group of coil springs and flat springs connected between the stationary lid and the movable lid.

5. A virtual well plate system according to claim 3, wherein the stationary lid includes at least one opening through which an external pressing device can be inserted for biasing the movable lid toward the base against the force of the at least one spring.

6. A virtual well plate system according to claim 2, wherein the base includes at least one upstanding side wall, and the at least one spring includes a plurality of coil springs connected between upper ends of the at least one side wall and the movable base.

7. A virtual well plate system according to claim 1, wherein the resistance arrangement includes a deformable spacer between said lid and said base.

8. A virtual well plate system according to claim 7, wherein the deformable spacer includes a resilient member.

9. A virtual well plate system according to claim 8, wherein the deformable spacer includes at least one spring positioned between the base and the lid.

10. A virtual well plate system according to claim 9, wherein the at least one spring is connected to one of the base and the lid.

11. A virtual well plate system according to claim 10, wherein each spring includes a cantilevered leaf spring connected to said one of the base and the lid.

12. A virtual well plate system according to claim 11, wherein the cantilevered leaf spring is positioned between the base and the lid when the lid is moved toward the base by the external force, so as to maintain the base plate and the lid plate separated by a predetermined distance sufficient to form the virtual wells.

13. A virtual well plate system according to claim 7, wherein the base includes a recess for receiving the deformable spacer.

14. A virtual well plate system according to claim 7, wherein the deformable spacer includes a non-resilient member.

15. A virtual well plate system according to claim 14, wherein the non-resilient member includes a crushable member which is crushed when the external force is applied to the lid.

16. A virtual well plate system according to claim 15, wherein the crushable member includes slits for permitting easy crushing thereof.

17. A virtual well plate system according to claim 1, wherein the base includes at least one peripheral flange having an upper surface, and the base plate includes an upper surface which is positioned lower than the upper surface of the at least one peripheral flange such that, when the lid is moved by the application of the external force sufficient to overcome the resistance of the resistance arrangement, the at least one peripheral flange maintains a lower surface of the lid plate at a predetermined distance from the upper surface of the base plate for formation of the virtual wells.

18. A virtual well plate system according to claim 17, wherein the at least one peripheral flange includes a recess in the upper surface thereof for holding said resistance arrangement and for permitting said resistance arrangement to collapse entirely in said recess such that the lid rests on the upper surface of the at least one peripheral flange when the lid is moved by the application of the external force sufficient to overcome the resistance of the resistance arrangement.

19. A virtual well plate system according to claim 1, wherein said base includes at least one upstanding side wall and said lid is slidably positioned within said at least one upstanding side wall.

20. A virtual well plate system according to claim 19, wherein said resistance arrangement includes at least one first detent on an inner surface of the at least one upstanding side wall and at least one second detent on an outer surface of said lid for engagement with said at least one first detent such that the at least one first detent supports the lid plate at a sufficient distance from the base plate to prevent formation of the virtual wells by the droplets thereon, and which permits movement of the lid plate toward the base plate upon application of an external force sufficient to overcome resistance of the at least one second detent riding over the at least one first detent in order to form the virtual wells by a combination of the droplets on the base plate and the lid plate.

21. A virtual well plate system according to claim 20, wherein there are two substantially vertically aligned first detents and one second detent which is captured between said two first detents to maintain the lid plate at a sufficient distance from the base plate to prevent formation of the virtual wells by the droplets thereon.

22. A virtual well plate system according to claim 1, wherein the resistance arrangement includes at least one laterally movable spring biased element mounted to the base for applying a lateral force to the lid to maintain the lid plate at a sufficient distance from the base plate to prevent formation of the virtual wells by the droplets thereon and to also laterally align the lid plate relative to the base plate, and which permits movement of the lid plate toward the base plate with said lateral alignment upon application of an external force sufficient to overcome resistance of the laterally movable spring biased element in order to form the virtual wells by a combination of the droplets on the base plate and the lid plate.

23. A virtual well plate system according to claim 22, wherein the base includes at least one upstanding side wall, and each laterally movable spring biased element includes a cam lever pivotally mounted to at least one upstanding side wall and a spring for biasing said cam lever inwardly of said base.

24. A virtual well plate system according to claim 23, wherein each said upstanding side wall to which at least one said cam lever is pivotally mounted includes at least one opening therein, and each said cam lever is pivotally mounted to the base and is positioned in a respective said opening.

25. A virtual well plate system according to claim 24, wherein each said cam lever includes a first detent on an inner facing surface thereof, and said lid includes at least one second detent on an outer facing surface thereof for engagement with each said first detent.

26. A virtual well plate system according to claim 22, wherein each laterally movable spring biased element includes a guide plate and at least one spring which biases the guide plate inwardly of the base in a lateral direction so as to maintain the base and lid in an assembled condition by the force of the guide plate on the lid such that the base plate and lid plate are maintained at a sufficient distance to prevent formation of the virtual wells by the droplets thereon, and which permits movement of the lid plate toward the base plate upon application of an external force sufficient to overcome frictional resistance between the guide plate and the lid in order to form the virtual wells by a combination of the droplets on the base plate and the lid plate.

27. A virtual well plate system according to claim 22, wherein the base includes at least one upstanding side wall, at least one upstanding side wall includes an opening therein, and each laterally movable spring biased element includes a cantilevered leaf spring hinged to the base and positioned in a respective said opening.

28. A virtual well plate system according to claim 27, further comprising a wedge element on an outer surface of the lid in association with each cantilevered leaf spring such that downward movement of the lid by the external force causes engagement between each cantilevered leaf spring and associated wedge to laterally move the lid plate into lateral alignment with the base plate.

29. A virtual well plate system according to claim 22, wherein each laterally movable spring biased element includes an upstanding cantilevered leaf spring having an inwardly bowed configuration and extending upwardly from said base between the upstanding side wall of the base and the lid for laterally biasing the lid when the lid plate is moved toward the base plate.

30. A virtual well plate system according to claim 22, wherein the base includes a plurality of connected upstanding side walls, and there are a plurality of said laterally movable spring biased elements mounted to two adjacent upstanding side walls for laterally aligning the lid relative to the base.

31. A virtual well plate system according to claim 1, further comprising at least one laterally movable spring biased element mounted to the base for applying a lateral force between the lid and the base to laterally align the lid plate relative to the base plate.

32. A virtual well plate system according to claim 31, wherein the at least one laterally movable spring biased element is provided at least one adjacent side walls of one of the following:

a) the lid, and

b) the base.

33. A virtual well plate system according to claim 31, wherein each laterally movable spring is selected from the group consisting of:

a) a coil spring,

b) a cantilevered spring, and

c) a biased pivoted member.