Nanoliter-scale synthesis of arrayed biomaterials and screening thereof
First Claim
Patent Images
1. A method of screening cell-polymer interactions, comprising:
- depositing monomers as a plurality of discrete elements on a substrate;
causing the deposited monomers to polymerize, thereby creating an array of discrete polymer elements on the substrate;
incubating the substrate in a cell-containing cell culture medium; and
characterizing a predetermined cell behavior on each polymer element.
3 Assignments
0 Petitions
Accused Products
Abstract
A method of screening cell-polymer interactions. The method includes depositing monomers as a plurality of discrete elements on a substrate, causing the deposited monomers to polymerize, thereby creating an array of discrete polymer elements on the substrate, incubating the substrate in a cell-containing culture medium, and characterizing a predetermined cell behavior on each polymer element.
-
Citations
61 Claims
-
1. A method of screening cell-polymer interactions, comprising:
- depositing monomers as a plurality of discrete elements on a substrate;
causing the deposited monomers to polymerize, thereby creating an array of discrete polymer elements on the substrate;
incubating the substrate in a cell-containing cell culture medium; and
characterizing a predetermined cell behavior on each polymer element. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
- depositing monomers as a plurality of discrete elements on a substrate;
-
20. A method of controlling cell behavior, comprising:
- selecting a first polymer in combination with which a predetermined cell exhibits a particular cell behavior;
selecting a second polymer differing from the first polymer in cross-link density or electron density; and
seeding the predetermined cell on the second polymer. - View Dependent Claims (21)
- selecting a first polymer in combination with which a predetermined cell exhibits a particular cell behavior;
-
22. A method of controlling a behavior of human embryonic stem cells, comprising:
exposing human embryonic stem cells to a synthetic polymer, wherein the polymer is selected to promote a predetermined behavior of the cells. - View Dependent Claims (24, 25, 26, 27)
-
23. A method of controlling a behavior of human embryonic stem cells, comprising:
exposing human embryonic stem cells to a synthetic polymer, wherein the polymer is not a polycation, polystyrene, a poly(lactide), or a co-polymer including lactide monomers.
-
28. A method of controlling cell behavior, comprising:
-
selecting a first monomer in combination with the polymer of which cells exhibit a particular cell behavior;
selecting a second monomer, that, when co-polymerized with the first monomer, modifies the cell behavior;
co-polymerizing the first and the second monomer to produce a co-polymer; and
seeding cells on the co-polymer. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
-
-
44. A method of controlling cell behavior, comprising:
-
selecting a first monomer in combination with the polymer of which cells exhibit a particular cell behavior;
selecting a growth factor that modifies the cell behavior when the cells are seeded on the polymer of the first monomer;
polymerizing the first monomer to produce a polymer; and
incubating the polymer in a cell-containing culture medium containing the growth factor. - View Dependent Claims (45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55)
-
-
56. A method of controlling cell behavior, comprising:
-
selecting cells characterized by a predetermined level of expression of a first gene;
selecting a monomer in combination with the polymer of which the cells exhibit a level of expression of the first gene different from the predetermined level;
polymerizing the monomer to produce a polymer; and
seeding the cells on the polymer. - View Dependent Claims (57)
-
-
58. A method of controlling cell behavior, comprising:
-
selecting cells characterized by a predetermined level of expression of a first protein;
selecting a monomer in combination with the polymer of which the cells exhibit a level of expression of the first protein different from the predetermined level;
polymerizing the monomer to produce a polymer; and
seeding the cells on the polymer. - View Dependent Claims (59, 60)
-
-
61. A method of supporting growth of C2C 12 cells in vitro, comprising culturing the C2C12 cells on a polymer produced from one or more of 1,4 butanediol dimethacrylate, diethylene glycol diacrylate, diethylene glycol dimethacrylate, 1,6-hexanediol diacrylate, neopentyl glycol diacrylate, phenylene diacrylate 1,3, propoxylated neopentyl glycol diacrylate, tetraethylene glycol diacrylate, tetraethylene glycol dimethacrylate, triethylene glycol diacrylate, triethylene glycol dimethacrylate, tripropylene glycol diacrylate, caprolactone 2-(methacryloyloxy)ethyl ester, 5-ethyl-5-(hydroxymethyl)-β
- ,β
-dimethyl-1,3-dioxane-2-ethanol diacrylate, 1,6-hexanediol propoxylate diacrylate, neopentyl glycol ethoxylate (1 EO/OH) diacrylate, trimethylolpropane benzoate diacrylate, tricyclo[5.2.1.02,6]decanedimethanol diacrylate,
- ,β
Specification