Compositions for isolating a cDNA encoding a membrane-bound protein
0 Assignments
0 Petitions
Accused Products
Abstract
A method for isolating a gene encoding a membrane-bound protein characterized by fusing a functional protein with a fused protein itself, which differs from the existing TMT method in which an epitope recognized by an antibody is carried in a fused protein. This method enabled selective isolation of a gene encoding a membrane-bound protein.
-
Citations
19 Claims
-
1. (Canceled)
-
12-16. -16. (Canceled)
-
17. A method for isolating or identifying a gene encoding a membrane-bound protein, the method comprising the steps of
(i) introducing into cells a vector comprising a sequence comprising (a) a DNA encoding a secretable protein and (b) a cDNA ligated downstream of the secretable protein-encoding DNA, wherein the secretable protein can bind to an antigen and is selected from the group consisting of an antibody, a fragment of an antibody, and a single-chain antibody; -
(ii) expressing in the cells a fusion protein encoded by the sequence comprising (a) and (b);
(iii) contacting the cells of (ii) with the antigen;
(iv) selecting a cell that binds to the antigen; and
(v) isolating or identifying the cDNA comprised in the vector that was introduced into the selected cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 18, 19)
-
Specification