Electron induced fluorescence for nucleic acid sequencing
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Abstract
An apparatus, compositions and related methods for sequencing a target nucleic acid are described. In certain embodiments, the apparatus is a microfluidic apparatus comprising an input chamber, microchannel, output chamber and a detection unit that is operatively connected to the microchannel. In preferred embodiments, the methods include hybridizing a target nucleic acid to one or more probe libraries, moving the hybridized target nucleic acid past the detector, and detecting bound probes. Probe libraries may comprise oligonucleotides or oligonucleotide analogs, preferably with each probe uniquely labeled. A linear order of labeled probes hybridized to the target nucleic acid can be detected and the target nucleic acid sequence deduced. In preferred embodiments, probe labels are detected by analysis of electron-induced fluorescence of probes labeled with conductive polymers.
35 Citations
26 Claims
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1-17. -17 (Cancelled)
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18. A nucleic acid sequencing apparatus comprising:
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a) an input chamber;
b) a microchannel in fluid communication with the input chamber; and
c) a detection unit operably coupled to the microchannel, the detection unit comprising an electron beam source and a detector. - View Dependent Claims (19, 20, 21, 22, 23, 24, 26)
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25. (Cancelled)
Specification