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Secretions of proteins by encapsulated cells

  • US 20050019839A1
  • Filed: 08/13/2004
  • Published: 01/27/2005
  • Est. Priority Date: 05/26/2001
  • Status: Active Grant
First Claim
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1. A method of analyzing a secreted protein, comprising:

  • encapsulating a cell in a microdrop wherein the microdrop comprises matrix component molecules, first biotin molecules linked to the matrix component molecules, capture molecules with affinity for a molecule secreted by the cell linked to second biotin molecules, and streptavidin linking the first and second biotin molecules, the first biotin molecules and the matrix component molecules being in a molar ratio of less than 0.85 moles biotin per mole matrix component molecules, wherein the molecule is secreted from the cell and binds to the capture molecules thereby being retained within the microdrop; and

    detecting the secreted molecule.

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