Nanoparticles for optical sensors

• US 20050019849A1
• Filed: 07/09/2004
• Published: 01/27/2005
• Est. Priority Date: 07/10/2003
• Status: Active Grant

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Oranth, Norbert, Desprez, Valerie, Spinke, Jurgen, Tusa, James Kenneth
• Granted Patent: US 7,427,491 B2
• Time in Patent Office: Days
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 33/521 Single-layer analytical elements

Abstract

The present invention concerns a polymer layer for a sensor, wherein the polymer layer has nanoparticles embedded therein which impart to the polymer layer recognizing properties as well as transducer properties, a sensor comprising such layer and the use of the sensor for detecting and/or quantifying a target analyte.

Claims

1. A sensor comprising a polymer layer, said polymer layer comprising recognizing and transducer properties, wherein

said recognizing properties are provided by a recognizing component,

said transducer properties are provided by a transducer, and

said recognizing component and said transducer are contained in one or more nanoparticles, said nanoparticles embedded in said polymer layer.

2. The sensor of claim 1, wherein said nanoparticles further comprise first or second nanoparticles, and wherein

said first nanoparticles comprise said recognizing component, and

said second nanoparticles comprise said transducer.

3. The sensor of claim 1, wherein said nanoparticles embedded in said polymer layer comprise both said recognizing component and said transducer.

4. The sensor of claim 1, wherein said recognizing component comprises a bioactive component.

5. The sensor of claim 4, wherein said bioactive component comprises an enzyme.

6. The sensor of claim 5, wherein said enzyme is selected from synthetic enzymes and gene-manipulated enzymes.

7. The sensor of claim 5, wherein said enzyme comprises an oxidative enzyme.

8. The sensor of claim 7, wherein said oxidative enzyme is selected from glucose oxidase, cholesterol oxidase, lactate oxidase, sarcosine oxidase, and mixtures thereof.

9. The sensor of claim 7, wherein said oxidative enzyme comprises glucose oxidase.

10. The sensor of claim 1, wherein said transducer comprises an optical transducer.

11. The sensor of claim 10, wherein said optical transducer comprises a luminescent dye.

12. The sensor of claim 11, wherein said luminescent dye is selected from [Ru(diphenylphenantroline)₃], octaethyl-Pt-porphyrin, octaethyl-Pt-porphyrin ketone, tetrabenzo-Pt-porphyrin, tetraphenyl-Pt-porphyrin, meso-tetraphenyl-tetrabenzo-Pt-porphyrin, tetracylcohexenyl-Pt-porphyrin, octaethyl-Pt-chlorine, tetraphenyl-Pt-chlorine, and mixtures thereof.

13. The sensor of claim 1, wherein said nanoparticles comprise a polymer material.

14. The sensor of claim 13, wherein said polymer material is selected from polyolefins, vinyl polymers, polyamides, polyesters, polyacetals, polycarbonates, polyurethanes, polysiloxanes, copolymers, and mixtures thereof.

15. The sensor of claim 1, wherein the mean diameter of said nanoparticles is between about 10 and about 500 nm.

16. The sensor of claim 1, wherein said polymer layer comprises a polymer selected from polyolefins, vinyl polymers, polyamides, polyesters, polyacetals, polycarbonates, polyurethanes, copolymers, and mixtures thereof.

17. The sensor of claim 1, wherein said polymer layer comprises one or more polyurethanes.

18. The sensor of claim 1, wherein said recognizing component and said transducer are entrapped within, conjugated to, or attached to one or more said nanoparticles embedded in said polymer layer.

19. The sensor of claim 1, wherein said sensor is used for detecting and/or quantifying a target analyte.

20. The sensor of claim 19, wherein said target analyte is selected from glucose, lactate, sarcosine, creatine, and mixtures thereof.

21. The sensor of claim 19, wherein said target analyte is glucose.

22. The sensor of claim 19, wherein said target analyte is contained within a body liquid sample.

23. A sensor comprising:

a light-transmissive substrate;

a polymer layer comprising recognizing and optical transducer properties, wherein

said recognizing properties are provided by a recognizing component,

said optical transducer properties are provided by an optical transducer,

said recognizing component and said optical transducer are contained in one or more nanoparticles,

said nanoparticles are embedded in said polymer layer, and

said polymer layer is deposited on said light-transmissive substrate; and

a membrane layer positioned on top of said polymer layer.

24. A polymer layer comprising recognizing and transducer properties, wherein

said recognizing properties are provided by a recognizing component,

said transducer properties are provided by a transducer, and

said recognizing component and said transducer are contained in one or more nanoparticles, said nanoparticles embedded in said polymer layer.

25. The polymer layer of claim 24, wherein said recognizing component is a bioactive component.

26. The polymer layer of claim 24, wherein the transducer is an optical transducer.

27. A process for producing a sensor comprising:

providing a light-transmissive substrate;

depositing a polymer layer on said substrate, wherein

said polymer layer comprises recognizing and transducer properties,

said recognizing properties are provided by a recognizing component,

said transducer properties are provided by a transducer, and

said recognizing component and said transducer are contained in one or more nanoparticles, said nanoparticles embedded in said polymer layer; and

coating a membrane layer on top of said polymer layer.

28. The process of claim 27, wherein said membrane layer comprises carbon black incorporated therein.