Miniaturized integrated nucleic acid processing and analysis device and method
First Claim
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1. A miniature device comprising:
- a body having a reaction chamber disposed therein;
a resistive heater electrically connected to a power source for applying power to said heater to facilitate a reaction in said reaction chamber;
a temperature sensor disposed on a surface of said body for determining a temperature within said reaction chamber; and
an appropriately programmed computer for monitoring said temperature and operating said power source to selectively apply said power to said heater.
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Abstract
A miniature device has a body including one, two or more reaction chambers. The reaction chambers are constructed for one or more of the following: sample acquisition, preparation or analysis. Preferably, a sample preparation reaction includes nucleic acid extraction, amplification, nucleic acid fragmentation, labeling, extension or a transcription.
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Citations
27 Claims
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1. A miniature device comprising:
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a body having a reaction chamber disposed therein;
a resistive heater electrically connected to a power source for applying power to said heater to facilitate a reaction in said reaction chamber;
a temperature sensor disposed on a surface of said body for determining a temperature within said reaction chamber; and
an appropriately programmed computer for monitoring said temperature and operating said power source to selectively apply said power to said heater. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A miniature device comprising:
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a body including at least two reaction chambers arranged in parallel, each said reaction chamber being constructed to separately receive a liquid;
each said reaction chamber being in fluid communication with a vent;
at least two resistive heaters electrically connected to a power source for applying power;
each said heater being constructed to deliver heat to one said reaction chamber;
at least two temperature sensors for determining separately a temperature within said reaction chambers; and
an appropriately programmed computer for monitoring said temperature and operating said power source to selectively apply said power to each said heater to facilitate separate reactions in said reaction chambers, wherein each said vent enables removal of gas from the corresponding reaction chamber thereby preventing a temperature variation in said liquid during said reaction. - View Dependent Claims (21, 22, 23, 24, 25, 26)
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27. A miniature device comprising:
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a body having a reaction chamber disposed therein and constructed to receive a liquid;
a vent in fluid communication with said reaction chamber;
a resistive heater electrically connected to a power source for applying power to said heater;
a temperature sensor disposed on a surface of said body for determining a temperature within said reaction chamber; and
an appropriately programmed computer for monitoring said temperature and operating said power source to selectively apply said power to said heater to facilitate a reaction in said reaction chamber, wherein said vent enables removal of gas from said reaction chamber thereby preventing a temperature variation in said liquid during said reaction.
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Specification