Spectroscopic method and apparatus for total hemoglobin measurement
First Claim
1. A method of measuring Tot-Hb in a sample, comprising, i) collecting an absorbance measurement of the sample using one or more than one first or second spectroscopic apparatus comprising a first primary calibration algorithm for one of Oxy-Hb, “
- Oxy-Hb plus Deoxy-Hb”
, or “
Total-Hb minus Met-Hb”
, and one or more than one second primary calibration algorithm for one or more than one of Met-Hb, Carboxy-Hb, or Sulf-Hb, or comprising a third primary calibration algorithm obtained by adding terms of the first primary calibration algorithm and the terms of the one or more than one second primary calibration algorithm together; and
ii) predicting either;
a) a first value for one of Oxy-Hb, “
Oxy-Hb plus Deoxy-Hb,”
or “
Total-Hb minus Met-Hb”
, in the sample by applying the first primary calibration algorithm to an order derivative of absorbance of the sample at one or more than one wavelength of a standard set of wavelengths, and predicting one or more than one second value for one or more than one of Met-Hb, Carboxy-Hb, or Sulf-Hb in the sample by applying the second primary calibration algorithm to an order derivative of absorbance at one or more than one wavelength of a standard set of wavelengths, and adding the first value and the one or more than one second value together to provide a measurement of Total-Hb;
or b) Total-Hb using the third primary calibration algorithm applied to an order derivative of absorbance of the sample at one or more than one wavelength of a standard set of wavelengths.
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Abstract
A method and apparatus for measuring Tot-Hb in a sample are provided. The method comprises collecting absorbance measurements of a sample using a spectroscopic apparatus that comprises a first primary calibration algorithm for one of Oxy-Hb, “Oxy-Hb plus Deoxy-Hb”, or “Total-Hb minus Met-Hb” and a second primary calibration algorithm for one or more than one of Met-Hb, Carboxy-Hb, or Sulf-Hb, or comprising a third primary calibration algorithm obtained by adding terms of the first primary calibration algorithm and the second primary calibration algorithm together. Followed by predicting either a first value for one of Oxy-Hb, “Oxy-Hb plus Deoxy-Hb,” or “Total-Hb minus Met-Hb” and predicting second value for one or more than one of Met-Hb, Carboxy-Hb, or Sulf-Hb in the sample and add the first and second value together, or predicting a value for Total-Hb.
80 Citations
32 Claims
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1. A method of measuring Tot-Hb in a sample, comprising,
i) collecting an absorbance measurement of the sample using one or more than one first or second spectroscopic apparatus comprising a first primary calibration algorithm for one of Oxy-Hb, “ - Oxy-Hb plus Deoxy-Hb”
, or “
Total-Hb minus Met-Hb”
, and one or more than one second primary calibration algorithm for one or more than one of Met-Hb, Carboxy-Hb, or Sulf-Hb, or comprising a third primary calibration algorithm obtained by adding terms of the first primary calibration algorithm and the terms of the one or more than one second primary calibration algorithm together; and
ii) predicting either;
a) a first value for one of Oxy-Hb, “
Oxy-Hb plus Deoxy-Hb,”
or “
Total-Hb minus Met-Hb”
, in the sample by applying the first primary calibration algorithm to an order derivative of absorbance of the sample at one or more than one wavelength of a standard set of wavelengths, and predicting one or more than one second value for one or more than one of Met-Hb, Carboxy-Hb, or Sulf-Hb in the sample by applying the second primary calibration algorithm to an order derivative of absorbance at one or more than one wavelength of a standard set of wavelengths, and adding the first value and the one or more than one second value together to provide a measurement of Total-Hb;
orb) Total-Hb using the third primary calibration algorithm applied to an order derivative of absorbance of the sample at one or more than one wavelength of a standard set of wavelengths. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 23)
- Oxy-Hb plus Deoxy-Hb”
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17. A reagentless spectroscopic method for measuring Total-Hb in a sample comprising:
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i) collecting an absorbance measurement of the sample using one or more than one spectroscopic apparatus comprising a primary calibration algorithm for Total-Hb, the sample having been exposed to atmospheric oxygen; and
ii) predicting a value for Total-Hb in the sample by applying the primary calibration algorithm to an order derivative of absorbance of the sample at one or more than one wavelength of a standard set of wavelengths, to provide a measurement of Total-Hb. - View Dependent Claims (18, 19, 20, 21, 22, 24, 25, 26, 27, 28, 29, 30)
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31. A spectroscopic apparatus, comprising:
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a) a source of electromagnetic radiation (EMR);
b) a first aperture located between the source of EMR and a sample slot to produce a light path therebetween;
c) the sample slot in the apparatus for receiving a sample vessel to be placed within the light path;
d) a second aperture located in the light path, between the sample slot and one or more than one photodetector, the one or more than one photodetector in operative association with the spectroscopic apparatus; and
eithere) a first primary calibration algorithm in operative association with the spectroscopic apparatus, the first primary calibration algorithm generated for one of Oxy-Hb, “
Oxy-Hb plus Deoxy-Hb,”
or “
Total-Hb minus Met-Hb”
, using an order derivative of absorbance at the one or more than one wavelength of a standard set of wavelengths obtained from one or more than one first apparatus using one or more than one calibration set having known reference values for one or more than one of Oxy-Hb, “
Oxy-Hb plus Deoxy-Hb”
, “
Total-Hb minus Met-Hb”
, Met-Hb, Carboxy-Hb, or Sulf-Hb, and a statistical technique, and one or more than one second primary calibration algorithm in operative association with the spectroscopic apparatus, the one or more than one second primary calibration algorithm generated for one or more than one of Met-Hb, Carboxy-Hb, or Sulf-Hb, using an order derivative of absorbance at the one or more than one wavelength of a standard set of wavelengths, the known reference values, and the statistical technique;
orf) a third primary calibration algorithm in operative association with the spectroscopic apparatus, the third primary calibration algorithm obtained by adding the terms of the first primary calibration algorithm and the terms of the one or more than one second primary calibration algorithm together.
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32. A spectroscopic apparatus, comprising:
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a) a source of electromagnetic radiation (EMR);
b) a first aperture located between the source of EMR and a sample slot to produce a light path therebetween;
c) the sample slot in the apparatus for receiving a sample vessel to be placed within the light path;
d) a second aperture located in the light path, between the sample slot and one or more than one photodetector, the one or more than one photodetector in operative association with the spectroscopic apparatus; and
e) one or more than one first primary calibration algorithm in operative association with the spectroscopic apparatus, the primary calibration algorithm for Total-Hb is generated using an order derivative of absorbance at one or more than one wavelength of a standard set of wavelengths obtained from one or more than one first apparatus, known reference values obtained from a primary calibration set, and a statistical technique, wherein each sample of the calibration set is exposed to atmospheric oxygen before spectroscopic measurement, and wherein the calibration set comprises known reference values from about 0 to about 100% of one or more than one of Oxy-Hb, Deoxy-Hb, Met-Hb, Carboxy-Hb, and Sulf-Hb.
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Specification