Detector and method for detecting weak fluorescent radiation with a microscope system
First Claim
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1. A detector for detecting weak fluorescent radiation comprising:
- a microscope system which defines a detected light beam;
a detection unit is provided for sensing individual photons of the detected light beam, wherein each photon is detected as one event and furnishes therefrom an output signal in the form of a characteristic function;
a filter circuit that forms a new characteristic function from the characteristic function; and
the filter circuit followed by a discriminator that distinguishes individual events on the basis of the new characteristic function and a threshold.
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Abstract
A detector for detecting weak fluorescent radiation with a microscope system (100) is disclosed. The microscope system (100) is configured in such a way that it senses individual photons of the detected light beam (17) each as one event (50), and furnishes therefrom an output signal in the form of a characteristic function (52). A filter circuit (61) forms, from the characteristic function (52), a new characteristic function (55) that is conveyed to a discriminator (60).
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Citations
9 Claims
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1. A detector for detecting weak fluorescent radiation comprising:
- a microscope system which defines a detected light beam;
a detection unit is provided for sensing individual photons of the detected light beam, wherein each photon is detected as one event and furnishes therefrom an output signal in the form of a characteristic function;
a filter circuit that forms a new characteristic function from the characteristic function; and
the filter circuit followed by a discriminator that distinguishes individual events on the basis of the new characteristic function and a threshold. - View Dependent Claims (2, 3, 4, 5)
- a microscope system which defines a detected light beam;
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6. A method for detecting weak fluorescent radiation with a microscope system that encompasses at least one detector, characterized by the following steps:
- conveying to a filter circuit a characteristic function, outputted by the detector, of one event;
generating a new characteristic function by an application of the characteristic function to an approximately mirrored characteristic function for correlation in the filter circuit;
conveying the new characteristic function to a discriminator; and
counting the events in a counter downstream from the discriminator. - View Dependent Claims (7, 8, 9)
- conveying to a filter circuit a characteristic function, outputted by the detector, of one event;
Specification