Detector and method for detecting weak fluorescent radiation with a microscope system

• US 20050024637A1
• Filed: 07/20/2004
• Published: 02/03/2005
• Est. Priority Date: 08/02/2003
• Status: Abandoned Application

• Current Assignee: Leica Microsystems Gmbh (Danaher Corporation)
• Original Assignee: Leica Microsystems Gmbh (Danaher Corporation)
• Inventors: Olschewski, Frank
• Application Number:
  US10/894,554
• Publication Number:
  US 20050024637A1
• Time in Patent Office: Days
• US Class Current: 356/318
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16
• CPC Class Codes: G02B 21/16 adapted for ultraviolet illumination ; Fluorescence microscopes G02B21/0076 takes precedence

Abstract

A detector for detecting weak fluorescent radiation with a microscope system (100) is disclosed. The microscope system (100) is configured in such a way that it senses individual photons of the detected light beam (17) each as one event (50), and furnishes therefrom an output signal in the form of a characteristic function (52). A filter circuit (61) forms, from the characteristic function (52), a new characteristic function (55) that is conveyed to a discriminator (60).

Claims

1. A detector for detecting weak fluorescent radiation comprising:

a microscope system which defines a detected light beam;

a detection unit is provided for sensing individual photons of the detected light beam, wherein each photon is detected as one event and furnishes therefrom an output signal in the form of a characteristic function;

a filter circuit that forms a new characteristic function from the characteristic function; and

the filter circuit followed by a discriminator that distinguishes individual events on the basis of the new characteristic function and a threshold.

2. The detector as defined in claim 1, wherein the filter circuit is embodied in the form of an analog electronic system.

3. The detector as defined in claim 1, wherein the filter circuit is constituted by a digital electronic system.

4. The detector as defined in claim 3, wherein the filter circuit coacts with a software program.

5. The detector as defined in claim 1, wherein the discriminator is followed by a counter that counts the events distinguished by the discriminator.

6. A method for detecting weak fluorescent radiation with a microscope system that encompasses at least one detector, characterized by the following steps:

conveying to a filter circuit a characteristic function, outputted by the detector, of one event;

generating a new characteristic function by an application of the characteristic function to an approximately mirrored characteristic function for correlation in the filter circuit;

conveying the new characteristic function to a discriminator; and

counting the events in a counter downstream from the discriminator.

7. The method as defined in claim 6, wherein the characteristic function outputted by the detector is shaped in the filter circuit;

and the filter circuit is analog.

8. The method as defined in claim 6, wherein the characteristic function outputted by the detector is shaped in the filter circuit;

and the filter circuit is digital.

9. The method as defined in claim 8, wherein the filter circuit coacts with a software program.