Imaging method and apparatus
First Claim
Patent Images
1. An apparatus for imaging, comprising:
- a light source emitting a polarized light beam;
an optical assembly including a control layer and a light reflection surface, wherein the light beam is passed through the control layer and reflected by the light reflection surface to provide an evanescent field with controlled height and intensity adjacent the light reflection surface, the light reflection surface being adapted to provide thereon a specimen array such that the specimen array in the evanescent field causes spatially distributed polarization changes in the cross-section of the light beam; and
a detector positioned to detect the spatially distributed polarization changes in the light beam to provide an image of the specimen array.
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Abstract
An imaging method and apparatus are provided, including a light source emitting a polarized light beam, and an optical assembly including a control layer and/or a light reflection surface. The control layer advantageously allows for control over the properties of a generated evanescent wave to optimize an image of a specimen array within the evanescent wave. The light reflection surface includes coupling means used to couple a receptor/capture agent advantageously allowing for flexible control over receptor specific regions.
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Citations
48 Claims
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1. An apparatus for imaging, comprising:
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a light source emitting a polarized light beam;
an optical assembly including a control layer and a light reflection surface, wherein the light beam is passed through the control layer and reflected by the light reflection surface to provide an evanescent field with controlled height and intensity adjacent the light reflection surface, the light reflection surface being adapted to provide thereon a specimen array such that the specimen array in the evanescent field causes spatially distributed polarization changes in the cross-section of the light beam; and
a detector positioned to detect the spatially distributed polarization changes in the light beam to provide an image of the specimen array. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. An apparatus for imaging, comprising:
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a light source emitting a polarized light beam;
an optical assembly including a light reflection surface, wherein the light beam is reflected by the light reflection surface to provide an evanescent field adjacent the light reflection surface, the light reflection surface including coupling means for providing thereon a specimen array such that the specimen array in the evanescent field causes spatially distributed polarization changes in the cross-section of the light beam; and
a detector positioned to detect the spatially distributed polarization changes in the light beam to provide an image of the specimen array. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method of imaging, comprising:
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passing a polarized light beam into an optical assembly including a control layer and a light reflection surface;
providing an evanescent field with controlled height and intensity adjacent the light reflection surface using the control layer;
providing a specimen array in the evanescent field causing spatially distributed polarization changes in the cross-section of the light beam; and
passing the reflected light beam out of the optical assembly where the spatially distributed polarization changes in the light beam are detected and processed to provide an image of the specimen array. - View Dependent Claims (21, 22, 23, 24, 25)
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26. A method of imaging, comprising:
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passing a polarized light beam into an optical assembly including a light reflection surface having coupling means for providing thereon an array of capture agents;
providing an evanescent field adjacent the light reflection surface;
providing molecules of interest such that the array of capture agents coupled with molecules of interest in the evanescent field cause spatially distributed polarization changes in the cross-section of the light beam; and
passing the reflected light beam out of the optical assembly where the spatially distributed polarization changes in the light beam are detected and processed to provide an image of the array of capture agents coupled with the molecules of interest. - View Dependent Claims (27, 28, 29, 30, 31)
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32. A cassette for use with an imaging apparatus, the cassette comprising:
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a light reflection surface including coupling means to allow formation thereon of a specimen array;
a control layer configured to direct a polarized light beam from a light source to the light reflection surface, such that a reflection of the polarized light beam at the light reflection surface generates a controlled evanescent field in the vicinity of the specimen array; and
a flow cell coupled to the light reflection surface, the flow cell including an inlet port and an outlet port for flowing analyte across the specimen array. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48)
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Specification