Biosensor and methods of use thereof
First Claim
1. A sensor element comprising a sensing electrode that reacts electrochemically with hydrogen peroxide to provide a detectable signal, the sensing electrode comprising platinum, graphite, a polymer matrix, and a geometric surface area that ranges from about 0.1 to about 3 cm2, wherein the electrode is affixed to a non-conductive surface, has a background current that ranges from about 2 to about 60 nA or less when measured in a buffer solution at an applied potential of 0.6V, and has a sensitivity of at least about 6 to about 180 nA/μ
- M of hydrogen peroxide when measured in a buffer solution at an applied potential of 0.6V.
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Accused Products
Abstract
An automated system for continual transdermal extraction of analytes present in a biological system is provided. The system can be used for detecting and/or measuring the concentration of the analyte using an electrochemical biosensor detection means. The system optionally uses reverse iontophoresis to carry out the continual transdermal extraction of the analytes.
231 Citations
70 Claims
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1. A sensor element comprising
a sensing electrode that reacts electrochemically with hydrogen peroxide to provide a detectable signal, the sensing electrode comprising platinum, graphite, a polymer matrix, and a geometric surface area that ranges from about 0.1 to about 3 cm2, wherein the electrode is affixed to a non-conductive surface, has a background current that ranges from about 2 to about 60 nA or less when measured in a buffer solution at an applied potential of 0.6V, and has a sensitivity of at least about 6 to about 180 nA/μ - M of hydrogen peroxide when measured in a buffer solution at an applied potential of 0.6V.
- View Dependent Claims (6, 12, 13, 57, 58, 59, 60, 61, 62, 63, 64, 65)
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2-5. -5. (Canceled)
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7-11. -11. (Canceled)
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14-23. -23. (Canceled)
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24. A method for monitoring an amount or concentration of an analyte present in a biological system, the method comprising
contacting the analyte with an enzyme that reacts with the analyte to produce hydrogen peroxide; -
detecting the hydrogen peroxide with a sensor element comprising a sensing electrode that reacts electrochemically with hydrogen peroxide to produce a detectable signal, the sensing electrode comprising platinum, graphite, a polymer matrix, and a geometric surface area that ranges from about 0.1 to about 3 cm2, wherein the electrode is affixed to a non-conductive surface, has a background current that ranges from about 2 to about 60 nA or less when measured in a buffer solution at an applied potential of 0.6V, and has a sensitivity of at least about 6 to about 180 nA/μ
M of hydrogen peroxide when measured in a buffer solution at an applied potential of 0.6V;
measuring the detectable signal, wherein the detectable signal is related to analyte amount or concentration in the biological system. - View Dependent Claims (26, 27, 33, 35, 66, 67, 68, 69, 70)
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25. (Canceled)
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28-32. -32. (Canceled)
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34. (Canceled)
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36-56. -56. (Canceled)
Specification