Rupestris stem pitting associated virus nucleic acids, proteins, and their uses

• US 20050032041A1
• Filed: 03/16/2004
• Published: 02/10/2005
• Est. Priority Date: 05/20/1997
• Status: Abandoned Application

• Current Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Original Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Inventors: Meng, Baozhong, Gonsalves, Dennis
• Application Number:
  US10/803,063
• Publication Number:
  US 20050032041A1
• Time in Patent Office: Days
• US Class Current: 435/5
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 15/8283 for virus resistance
• CPC Class Codes: C12N 2770/26022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12Q 1/701 Specific hybridization probes
• CPC Class Codes: Y10S 424/801 involving antibody or fragment thereof produced by recombinant dna technology

Abstract

The present invention relates to an isolated protein or polypeptide corresponding to a protein or polypeptide of a Rupestris stem pitting associated virus. The encoding DNA molecule, either alone in isolated form, in an expression system, a host cell, or a transgenic grape plant, is also disclosed. Other aspects of the present invention relate to a method of imparting Rupestris stem pitting associated virus resistance to grape plants by transforming them with the DNA molecule of the present invention, and a method of detecting the presence of a Rupestris stem pitting associated virus, such as RSPaV-1, in a sample.

Claims

1. An isolated protein or polypeptide corresponding to a protein or polypeptide of a Rupestris stem pitting associated virus.

18. An isolated DNA molecule encoding a protein or polypeptide according to claim 1.

35. A host cell transformed with a heterologous DNA molecule according to claim 18.

36. The host cell according to claim 35, wherein the host cell is selected from a group consisting of Agrobacterium vitis and Agrobacterium tumefaciens.

37. The host cell according to claim 35, wherein the host cell is a grape cell.

38. The host cell according to claim 35, wherein the protein or polypeptide is selected from a group consisting of a replicase, a coat protein, and a protein of a triple gene block.

39. A transgenic Vitis scion cultivar or rootstock cultivar comprising the DNA molecule according to claim 18.

40. A transgenic Vitis scion cultivar or rootstock cultivar according to claim 39, wherein the protein or polypeptide is selected from a group consisting of a replicase, a coat protein, and a protein of a triple gene block.

2-17. -17. (cancelled)

19-34. -34. (cancelled)

41-63. -63. (cancelled).