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Methods of making nucleic acid molecules with reduced secondary structure

  • US 20050032055A1
  • Filed: 04/25/2003
  • Published: 02/10/2005
  • Est. Priority Date: 07/20/1999
  • Status: Abandoned Application
First Claim
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1. A method of synthesizing an unstructured nucleic acid with reduced secondary structure relative to a nucleic acid of substantially identical nucleotide sequence having naturally occurring bases, the method comprising steps of:

  • providing a template nucleic acid strand comprising at least two self-complementary sequence elements;

    selecting a DNA polymerase for use in synthesizing a new unstructured nucleic acid strand complementary to the template nucleic acid strand so that the new unstructured strand contains at least two self-complementary sequence elements;

    providing a collection of nucleotides that are substrates for the DNA polymerase, the collection being selected to allow for intermolecular base-pair interactions but not intermolecular base pair interactions; and

    allowing extension by the DNA polymerase, so that the new unstructured nucleic acid strand is synthesized and the self-complementary sequence elements within the unstructured nucleic acid strand do not form intramolecular hybrids with one another but do form intermolecular hybrids with the template strand.

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