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Methods and apparatus for use in detection and quantitation of various cell types and use of optical bio-disc for performing same

  • US 20050032126A1
  • Filed: 03/02/2004
  • Published: 02/10/2005
  • Est. Priority Date: 03/03/2003
  • Status: Abandoned Application
First Claim
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1. A method of detecting and quantifying a cell type of interest among various types of cells, said method comprising the steps of:

  • providing a test sample having target cells, said target cells having a common surface marker, said target cells including a first cell type having a first surface marker and a second cell type having a second surface marker;

    providing a bio-disc, said bio-disc comprising a substantially circular substrate having a center and an outer edge, a target zone disposed between said center and said outer edge, a plurality of capture antibodies bound to said substantially circular substrate within said target zone such that said plurality of capture antibodies are immobilized in said target zone, said plurality of capture antibodies having affinity for said common surface marker on said target cells;

    depositing said test sample on said target zone;

    allowing said target cells including said first and second type cells present in said test sample to bind with said plurality of capture antibodies through said common surface marker;

    washing said target zone to remove any unbound cells;

    providing a reporter agent having a signal antibody bound thereto, said signal antibody having specific affinity to said first surface marker;

    depositing said reporter agent on said target zone;

    allowing said signal antibody to bind to said first surface marker on said first cell type in said target zone to thereby tag said first cell type;

    washing the target zone to remove any unbound reporter agent; and

    scanning a beam of electromagnetic radiation over said target zone to thereby determine the number of tagged first cell type cells and untagged second cell type cells.

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