Methods for humanizing rabbit monoclonal antibodies
First Claim
1. A method of humanizing a rabbit monoclonal antibody, said method comprising:
- (a) comparing an amino acid sequences of a heavy an a light chain variable domain of a parent rabbit antibody to the amino acid sequence of a heavy and a light chain variable domain of a similar human antibody; and
(b) altering amino acids within the framework regions of said heavy and light chain variable domains of said rabbit antibody such that the altered framework regions are more similar in sequence to the equivalent framework regions of said similar human antibody;
wherein said altered amino acids are not involved in complementarity determining region (CDR) contacts, interchain contacts, or are buried residues with substantially different side chains.
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Abstract
The invention provides a method for humanizing a rabbit monoclonal antibody. In general, the method involves comparing an amino acid sequences of a parent rabbit antibody to the amino acid sequences of a similar human antibody, and altering the amino acid sequence of the parent rabbit antibody such its framework regions are more similar in sequence to the equivalent framework regions of the similar human antibody. In many embodiments, amino acids in the parent rabbit antibody that are not CDR contact residues, interchain contact residues, or buried residues, are not modified. The invention further provides nucleic acids encoding the subject antibodies, as well as vectors and host cells comprising the nucleic acids and methods for producing a subject antibody. The subject antibodies, nucleic acid compositions and kits find use in a variety of applications, including diagnostics and therapeutic treatment and research of conditions and diseases.
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Citations
20 Claims
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1. A method of humanizing a rabbit monoclonal antibody, said method comprising:
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(a) comparing an amino acid sequences of a heavy an a light chain variable domain of a parent rabbit antibody to the amino acid sequence of a heavy and a light chain variable domain of a similar human antibody; and
(b) altering amino acids within the framework regions of said heavy and light chain variable domains of said rabbit antibody such that the altered framework regions are more similar in sequence to the equivalent framework regions of said similar human antibody;
wherein said altered amino acids are not involved in complementarity determining region (CDR) contacts, interchain contacts, or are buried residues with substantially different side chains. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification