Method for optical coherence tomography imaging with molecular contrast

• US 20050036150A1
• Filed: 01/26/2004
• Published: 02/17/2005
• Est. Priority Date: 01/24/2003
• Status: Active Grant

• Current Assignee: Duke University
• Original Assignee: Duke University
• Inventors: Izatt, Joseph A., Yang, Changhuei, Yazdanfar, Siavash, Rollins, Andrew M., Applegate, Brian E., Rao, Divakar K., Choma, Michael A.
• Granted Patent: US 7,075,658 B2
• Time in Patent Office: Days
• US Class Current: 356/479
• CPC Class Codes: G01B 2290/70 Using polarization in the interferometer
• CPC Class Codes: G01B 9/02002 using two or more frequencies
• CPC Class Codes: G01B 9/02004 using frequency scans
• CPC Class Codes: G01B 9/02007 Two or more frequencies or sources used for interferometric measurement using only beat G01B9/02003
• CPC Class Codes: G01B 9/02014 by using pulsed light
• CPC Class Codes: G01B 9/02044 Imaging in the frequency domain, e.g. by using a spectrometer
• CPC Class Codes: G01B 9/02069 Synchronization of light source or manipulator and detector
• CPC Class Codes: G01B 9/02091 Tomographic interferometers, e.g. based on optical coherence

Abstract

Spatial information, such as concentration and displacement, about a specific molecular contrast agent, may be determined by stimulating a sample containing the agent, thereby altering an optical property of the agent. A plurality of optical coherence tomography (OCT) images may be acquired, at least some of which are acquired at different stimulus intensities. The acquired images are used to profile the molecular contrast agent concentration distribution of the sample.

Claims

1. A method of determining spatial information about specific molecular contrast agents in a sample comprising the steps of:

(a) stimulating, at a plurality of intensity levels, the sample thereby altering an optical property of the molecular contrast agent;

(b) acquiring a plurality of optical coherence tomography (OCT) images, at least some of the plurality of OCT images being acquired at different stimulus intensities; and

(c) profiling the molecular contrast agent concentration distribution based on the plurality of OCT images.

2. The method of claim 1, wherein the stimulation intensity levels provide transient changes in the optical property of the molecular contrast agent.

3. The method of claim 1, wherein at least some of the stimulation intensity levels provide saturation of the optical property of the molecular contrast agent.

4. The method of claim 1, wherein stimulating the molecular contrast agent increases the optical absorption of the agent.

5. The method of claim 1, wherein stimulating the molecular contrast agent increases the optical transparency of the agent.

6. The method of claim 1, wherein stimulating the sample is performed using light at one wavelength and acquiring the images is performed using light at another wavelength.

7. The method of claim 1, wherein stimulating the sample is performed using light at the same wavelength as the light used for acquiring the images.

8. The method of claim 7, wherein the phase of light used for stimulating the sample is different that the phase of light used for acquiring the images.

9. The method of claim 1, wherein acquiring the images is performed after stimulating the sample.

10. The method of claim 1, wherein acquiring the images is performed while stimulating the sample.

11. The method of claim 1, wherein profiling the molecular contrast agent concentration includes comparing OCT images acquired with the stimulation source active to those acquired with the stimulation source inactive.

12. The method of claim 1, wherein profiling the molecular contrast agent concentration includes comparing OCT images acquired at different intensities of stimulation.