Biochemical detecting device for magnetic beads and method using the same

• US 20050036908A1
• Filed: 08/13/2004
• Published: 02/17/2005
• Est. Priority Date: 08/15/2003
• Status: Abandoned Application

• Current Assignee: National Tsing Hua University, Precision Instrument Development Center
• Original Assignee: National Tsing Hua University, Precision Instrument Development Center
• Inventors: Lin, Ming-Yu, Yang, Jing-Tang, Yu, Chih-Sheng, Chou, Hsiao-Yu, Chen, Chien-Jen, Hu, Yi-Chiuen, Yuan, Shih-Jun
• Application Number:
  US10/917,762
• Publication Number:
  US 20050036908A1
• Time in Patent Office: Days
• US Class Current: 422/58
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 33/54326 Magnetic particles

Abstract

A biochemical detecting device for separating a reagent, a plurality of magnetic beads and a target from a mixture, and detecting the target is provided. The biochemical detecting device includes a first substrate, at least two first electrode sets located on the first substrate, a second electrode set located on the first substrate and between the two first electrode sets, and a second substrate covering the first substrate, each of the first electrode sets and the second electrode set. Accordingly, the movement of the mixture is digitally controlled by the provided biological detecting device.

Claims

1. A biochemical detecting device for separating a reagent, a plurality of magnetic beads and a target from a mixture, and detecting said target, comprising:

a first substrate;

at least two first electrode sets located on said first substrate;

a second electrode set located on said first substrate and between said two first electrode sets; and

a second substrate covering said first substrate, each of said first electrode sets and said second electrode set, wherein a movement of said mixture is digitally controlled.

2. The biochemical detecting device according to claim 1, wherein said reagent and said plurality of magnetic beads move and interact between said first substrate and said second substrate when a first current is applied to said first electrode sets;

said plurality magnetic beads are attracted by said second electrode set, and said target is identified and detected through a fluorescence signal of said reagent attached on said target when a second current is applied to said second electrode set; and

a certain reaction of said target is initiated to generate a current signal to be detected when a third current is applied to one of said first electrode sets.

3. The biochemical detecting device according to claim 1, wherein said target is a mixture of blood and a dye agent.

4. The biochemical detecting device according to claim 1, wherein said first substrate and said second substrate are silicon substrates.

5. The biochemical detecting device according to claim 1, wherein said first substrate and said second substrate are glass substrates.

6. The biochemical detecting device according to claim 1, wherein said first electrode set and said second electrode set are electromagnetic coils.

7. The biochemical detecting device according to claim 1, wherein said first electrode set and said second electrode set are inductors.

8. The biochemical detecting device according to claim 1, wherein each of said current signal and said fluorescence signal is forwarded by said first electrode set to be detected.

9. A biochemical detecting method for detecting a target in a plurality of magnetic beads of a liquid drop by a biochemical detecting device, comprising steps of:

mixing a target, a reagent having a fluorescence signal, and a plurality of magnetic beads to form a fluid, wherein said target is adsorbed by said plurality of magnetic beads;

driving said fluid by a first electrode set of said biochemical detecting device such that said fluid moves to a second electrode set of said biological detecting device;

attracting said plurality magnetic beads by said second electrode set; and

identifying and detecting said fluorescence signal of said reagent adsorbed on said target.

10. The biochemical detecting method according to claim 9, wherein said biochemical detecting device further comprises:

a first substrate;

at least two first electrode sets located on said first substrate;

a second electrode set located on said first substrate and between said two first electrode sets; and

a second substrate covering said first substrate, each of said first electrode sets and said second electrode set.

11. The biochemical detecting method according to claim 10, wherein said reagent and said plurality of magnetic beads are able to move and interact between said first substrate and said second substrate when a first current is applied to said first electrode sets;

said plurality magnetic beads are attracted by said second electrode set, and said target is identified and detected through a fluorescence signal of said reagent attached on said target when a second current is applied to said second electrode set; and

a certain reaction of said target is initiated to generate a current signal to be detected when a third current is applied to one of said first electrode sets.

12. A biochemical detecting method for detecting a target in a plurality of magnetic beads of a liquid drop by a biochemical detecting device, comprising steps of:

mixing a target, a reagent and a plurality of magnetic beads to form a fluid, wherein said target is adsorbed by said plurality of magnetic beads;

driving said fluid by a first electrode set of said biochemical detecting device such that said fluid moves to a second electrode set of said biological detecting device;

attracting said plurality magnetic beads by said second electrode set; and

identifying and detecting said current signal of said reagent adsorbed on said target.

13. The biochemical detecting method according to claim 12, wherein said biochemical detecting device comprises:

a first substrate;

at least two first electrode sets located on said first substrate;

a second electrode set located on said first substrate and between said two first electrode sets; and

a second substrate covering said first substrate, each of said first electrode sets and said second electrode set.

14. The biochemical detecting method according to claim 13, wherein said reagent and said plurality of magnetic beads are able to move and interact between said first substrate and said second substrate when a first current is applied to said first electrode sets;

said plurality magnetic beads are attracted by said second electrode set, and said target is identified and detected through a fluorescence signal of said reagent attached on said target when a second current is applied to said second electrode set; and

a certain reaction of said target is initiated to generate a current signal to be detected when a third current is applied to one of said first electrode sets.