Methods and apparatuses for electronic determination of analytes
First Claim
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1. A method for determining analytes, which comprises the following steps:
- (a) providing an apparatus comprising (i) a light source matrix, (ii) a microfluidic support having channels which contain a plurality of predetermined areas at which in each case different receptors are immobilized on the support, (iii) means for supplying fluids to the support and for discharging fluids from the support and (iv) an electronic detection matrix having a plurality of electrodes assigned to the predetermined areas containing immobilized receptors on the support, (b) contacting the support with a sample containing analytes and (c) determining the analytes by electronic detection via binding thereof to the receptors immobilized on the support.
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Abstract
The invention relates to a method and an apparatus for determining analytes by electronic detection using a microfluidic support.
48 Citations
24 Claims
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1. A method for determining analytes, which comprises the following steps:
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(a) providing an apparatus comprising (i) a light source matrix, (ii) a microfluidic support having channels which contain a plurality of predetermined areas at which in each case different receptors are immobilized on the support, (iii) means for supplying fluids to the support and for discharging fluids from the support and (iv) an electronic detection matrix having a plurality of electrodes assigned to the predetermined areas containing immobilized receptors on the support, (b) contacting the support with a sample containing analytes and (c) determining the analytes by electronic detection via binding thereof to the receptors immobilized on the support. - View Dependent Claims (2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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3. The method as claimed in 1, wherein as microfluidic support with closed channels is used.
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21. An apparatus for determining analytes, which comprises
(i) a light source matrix, (ii) a support containing a plurality of predetermined positions at which in each case different receptors are immobilized on the support, (iii) means for supplying fluids to the support and for discharging fluids from the support and (iv) an electronic detection matrix having a plurality of electrodes assigned to the predetermined positions containing immobilized receptors on the support.
Specification