Optical bio-discs including spiral fluidic circuits for performing assays
First Claim
1. An optical bio-disc, comprising:
- a cap portion having inlet and vent ports formed therein;
a first channel layer having cut-out portions;
a second channel layer having cut-out portions;
a third channel layer having cut-out portions;
a fourth channel layer having cut-out portions; and
a substantially circular substrate having a center and an outer edge, wherein the substrate is configured to support the cap portion, the first channel layer, the second channel layer, the third channel layer, and the fourth channel layer.
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Accused Products
Abstract
The present invention relates to methods and apparatus for assays including optical bio-discs with spiral fluidic circuits and related detection systems. The optical bio-disc 110 includes a cap portion 116 having inlet and vent ports formed therein, a first channel layer 632 having cut-out portions, a second channel layer 634 having cut-out portions; a third channel layer 636 having cut-cut portions, a fourth channel layer 638 having cut-out portions, and a substantially circular substrate having a center and an outer edge. The cut-out portions are in register with each other such that when the bio-disc 110 is assembled a spiral fluidic circuit is formed having an inlet port, a mixing chamber 134, upper flow chambers 620, lower pass through chambers 622, inlet passages 626, outlet passages 628, a circumferential analysis chamber 618, and vent ports in fluid communication.
78 Citations
24 Claims
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1. An optical bio-disc, comprising:
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a cap portion having inlet and vent ports formed therein;
a first channel layer having cut-out portions;
a second channel layer having cut-out portions;
a third channel layer having cut-out portions;
a fourth channel layer having cut-out portions; and
a substantially circular substrate having a center and an outer edge, wherein the substrate is configured to support the cap portion, the first channel layer, the second channel layer, the third channel layer, and the fourth channel layer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method of making a chromatographic optical bio-disc, said method comprising the steps of:
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providing a substrate having a center and an outer edge;
providing a cap portion having an inlet port and a vent port formed therein;
providing a first channel layer having cut-out portions;
providing a second channel layer having cut-out portions;
providing a third channel layer having cut-out portions;
providing a fourth channel layer having cut-out portions; and
assembling the optical bio-disc such that said cap portion and said channel layers are supported by the substrate and said cut-out portions form a spiral fluidic circuit. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method of using an optical bio-disc, the method comprising:
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depositing a test sample into the bio-disc through an inlet port;
rotating said bio-disc at a predetermined speed and for a predetermined period of time to allow said test sample to move through a bio-matrix pad so that analytes present in the sample bind to capture agents in the bio-matrix pad;
continuing said rotating step to thereby move said test sample through a spiral fluidic circuit of the optical bio-disc and into an analysis chamber;
depositing signal agents having one or more reporters attached thereto into the bio-disc through said inlet port;
rotating said disc to cause said signal agents to move through said bio-matrix pad so that said signal agents bind to any analyte that is bound to the capture agents in the bio-matrix pad; and
scanning the bio-matrix pads located in the inlet and outlet passages with a beam of electromagnetic radiation to determine the presence and amount of signal agents bound to the analytes within the bio-matrix pads. - View Dependent Claims (21, 22, 23, 24)
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Specification