Methods and kits for assays of rapid screening of diabetes
First Claim
1. A method for rapidly screening for diabetes, comprising the steps of:
- contacting a glucose-sensing ophthalmic device with an ocular fluid, wherein the glucose-sensing ophthalmic device comprises a testing agent composition which specifically and reversibly interacts with glucose to form a detectable signal which changes in a concentration-dependent manner;
determining by means of the glucose-sensing ophthalmic device a first glucose concentration in the ocular fluid;
administering orally a load of carbohydrate to the patient;
at a period of time of less than 50 minutes after orally administering of the load of carbohydrate, determining by means of the glucose-sensing ophthalmic device a second glucose concentration in the ocular fluid; and
comparing the second glucose concentration with the first glucose concentration to determine if the patient is likely to be a diabetic.
0 Assignments
0 Petitions
Accused Products
Abstract
The invention provides an in vivo screening assay and an in vitro screening assay for rapid screening of diabetes. A method of the invention includes determining a first glucose concentration in an ocular fluid of a patient; administering orally a load of carbohydrate to the patient; determining a second glucose concentration in an ocular fluid of the patient at a period of time of less than 50 minutes after orally administering of the load of carbohydrate; comparing the second glucose concentration with the first glucose concentration to determine if the patient is likely to be a diabetic. The method of the invention is performed by using a kit of the invention. The kit comprises: (1) a glucose-sensing ophthalmic device and instructions for using the glucose-sensing ophthalmic device to screen for diabetes; or (2) two or more tear-collecting devices, and a testing agent composition which specifically reacts with glucose to form a detectable signal. The glucose-sensing ophthalmic device comprises a testing agent composition which specifically and reversibly interacts with glucose to form a detectable optical signal which changes in a concentration-dependent manner.
160 Citations
30 Claims
-
1. A method for rapidly screening for diabetes, comprising the steps of:
-
contacting a glucose-sensing ophthalmic device with an ocular fluid, wherein the glucose-sensing ophthalmic device comprises a testing agent composition which specifically and reversibly interacts with glucose to form a detectable signal which changes in a concentration-dependent manner;
determining by means of the glucose-sensing ophthalmic device a first glucose concentration in the ocular fluid;
administering orally a load of carbohydrate to the patient;
at a period of time of less than 50 minutes after orally administering of the load of carbohydrate, determining by means of the glucose-sensing ophthalmic device a second glucose concentration in the ocular fluid; and
comparing the second glucose concentration with the first glucose concentration to determine if the patient is likely to be a diabetic. - View Dependent Claims (2, 3, 4, 5, 6)
-
-
7. A method for rapidly screening for diabetes, comprising the steps of:
-
collecting a first tear fluid from a patient using a first tear-collecting device;
assaying a specific amount of the first tear fluid to determine a first glucose concentration;
administering orally a load of carbohydrate to the patient;
collecting a second tear fluid, at a period of time of less than 50 minutes after orally administering of the load of carbohydrate, using a second tear-collecting device;
assaying a specific amount of the second tear fluid to determine a second glucose concentration; and
comparing the second glucose concentration with the first glucose concentration to determine if the patient is likely to be a diabetic. - View Dependent Claims (8, 9, 10, 11)
-
-
12. A kit for rapid screening of diabetes, the kit comprising:
- a glucose-sensing ophthalmic device and instructions for using the glucose-sensing ophthalmic device to screen for diabetes, wherein the glucose-sensing ophthalmic device comprises a testing agent composition which specifically and reversibly interacts with glucose to form a detectable optical signal which changes in a concentration-dependent manner.
- View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21)
-
22. A kit for rapid screening of diabetes, the kit comprising:
- two or more tear-collecting devices, and a testing agent composition which specifically reacts with glucose to form a detectable signal.
- View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30)
Specification