Hookworm vaccine

• US 20050042232A1
• Filed: 04/16/2004
• Published: 02/24/2005
• Est. Priority Date: 10/17/2001
• Status: Active Grant

• Current Assignee: George Washington University
• Original Assignee: George Washington University
• Inventors: Wang, Yan, Jones, Brian, Goud, Gaddam, Bottazzi, Maria Elena, Loukas, Alexander, Bethony, Jeffrey, Hotez, Peter, Zhan, Bin, Bdamchian, Mahnaz, Mendez, Susana, Hawdon, John, Williamson, Angela, Ashcom, James
• Granted Patent: US 7,303,752 B2
• Time in Patent Office: Days
• US Class Current: 424/191.100
• CPC Class Codes: A61K 2039/55577 Saponins; Quil A; QS21; ISCOMS
• CPC Class Codes: A61K 39/0003 Invertebrate antigens
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Preparations which elicit an immune response to hookworm antigens and which may be utilized as hookworm vaccines are provided. In addition, a method of increasing the effectiveness of vaccinations against infectious diseases in patients infected with hookworm is provided. The method involves chemically treating the hookworm infestation prior to administering the vaccine.

Claims

1-33. -33. (cancelled):

34. A method for enabling vaccination of a patient against infectious diseases, comprising the steps of:

a) treating hookworm infection to a degree sufficient to increase lymphocyte proliferation; and

b) vaccinating said patient against said infectious disease.

35. The method of claim 34 wherein said infectious disease is selected from the group consisting of HIV, tuberculosis, malaria, measles, tetanus, diphtheria, pertussis, and polio.

36. A method for enabling hookworm vaccination, comprising the steps of:

a) chemically treating a hookworm infected patient to ameliorate hookworm infection; and

b) vaccinating said patient with a recombinant or synthetic antigen or fragment thereof derived from hookworm after amelioration of hookworm infection.

37-97. -97. (Cancelled)

98. A composition comprising:

a cocktail of recombinant or synthetic antigens derived from hookworm, and,

a pharmacologically acceptable carrier.

99. The composition of claim 98, wherein said composition comprises at least one larval stage antigen and at least one adult stage antigen.

100. The composition of claim 98, wherein said antigen is ASP-1, ASP-2, MTP-1, 103 (SAA), 16, GST or an antigen having at least 80% homology therewith.

101. The composition of claim 98, wherein said antigen is selected from the group consisting of GST, CP-2, APR-1, APR-2, MEP-1, TMP or an antigen having at least 80% homology therewith.

102. The composition of claim 98, wherein a species of said hookworm is selected from the group consisting of Necator americanus, Ancylostoma caninum, Ancylostoma ceylanicum, and Ancyclostoma duodenale.

103. A method of vaccinating or eliciting an immune response to hookworm in a mammal, comprising the step of,

administering to said mammal an effective amount of a composition comprising a recombinant or synthetic antigen derived from hookworm, and

a pharmacologically acceptable carrier.

104. The method of claim 103 wherein said composition includes

a cocktail of recombinant or synthetic antigens derived from hookworm, and,

a pharmacologically acceptable carrier.

105. The method of claim 103, wherein said composition comprises at least one larval stage antigen and at least one adult stage antigen.

106. The method of claim 103, wherein said antigen is ASP-1, ASP-2, MTP-1, 103 (SAA), 16, GST, or an antigen having at least 80% homology therewith.

107. The method of claim 103, wherein said antigen is selected from the group consisting of GST, CP-2, APR-1, APR-2, MEP-1, TMP, or an antigen having at least 80% homology therewith.

108. The method of claim 103, wherein a species of said hookworm is selected from the group consisting of Necator americanus, Ancylostoma caninum, Ancylostoma ceylanicum, and Ancyclostoma duodenale.

109. The method of claim 103, further comprising the step of chemically treating a hookworm-infected patient prior to said step of administering.

110. A method of reducing blood loss in a patient infected with hookworm, comprising the step of

administering to said patient an effective amount of a composition comprising a recombinant or synthetic antigen derived from hookworm, and

a pharmacologically acceptable carrier.

111. The method of claim 110 wherein said composition includes

a cocktail of recombinant or synthetic antigens derived from hookworm, and,

a pharmacologically acceptable carrier.

112. The method of claim 110, wherein said composition comprises at least one larval stage antigen and at least one adult stage antigen.

113. The method of claim 110, wherein said antigen is ASP-1, ASP-2, MTP-1, 103 (SAA), 16, GST, or an antigen having at least 80% homology therewith.

114. The method of claim 110, wherein said antigen is selected from the group consisting of GST, CP-2, APR-1, APR-2, MEP-1, TMP, or an antigen having at least 80% homology therewith.

115. The method of claim 110, wherein a species of said hookworm is selected from the group consisting of Necator americanus, Ancylostoma caninum, Ancylostoma ceylanicum, and Ancyclostoma duodenale.

116. The method of claim 110, further comprising the step of chemically treating a hookworm-infected patient prior to said step of administering.

117. A method of reducing hookworm size, or quantitative egg count or hookworm burden in a patient infected with hookworm, comprising the step of administering to said mammal an effective amount of a composition comprising

a recombinant or synthetic antigen derived from hookworm, and

a pharmacologically acceptable carrier.

18. The method of claim 117 wherein said composition includes

a cocktail of recombinant or synthetic antigens derived from hookworm, and,

a pharmacologically acceptable carrier.

19. The method of claim 117, wherein said composition comprises at least one larval stage antigen and at least one adult stage antigen.

120. The method of claim 117, wherein said antigen is ASP-1, ASP-2, MTP-1, 103, 16, GST, or an antigen having at least 80% homology therewith.

121. The method of claim 117, wherein said antigen is selected from the group consisting of GST, CP-2, APR-1, APR-2, MEP-1, TMP, or an antigen having at least 80% homology therewith.

122. The method of claim 117, wherein a species of said hookworm is selected from the group consisting of Necator americanus, Ancylostoma caninum, Ancylostoma ceylanicum, and Ancyclostoma duodenale.

123. The method of claim 117, further comprising the step of chemically treating a hookworm-infected patient prior to said step of administering.

124. A method of decreasing L3 migration across skin of a mammal, comprising the step of

administering to said mammal an effective amount of a composition comprising

a recombinant or synthetic antigen derived from hookworm, and

a pharmacologically acceptable carrier.

125. The method of claim 124 wherein said composition includes a cocktail of recombinant or synthetic antigens derived from hookworm, and, a pharmacologically acceptable carrier.

126. The method of claim 124, wherein said composition comprises at least one larval stage antigen and at least one adult stage antigen.

127. The method of claim 124, wherein said antigen is ASP-1, ASP-2, MTP-1, 103 (SAA), 16, GST, or an antigen having at least 80% homology therewith.

128. The method of claim 124, wherein said antigen is selected from the group consisting of GST, CP-2, APR-1, APR-2, MEP-1, TMP, or an antigen having at least 80% homology therewith.

129. The method of claim 124, wherein a species of said hookworm is selected from the group consisting of Necator americanus, Ancylostoma caninum, Ancylostoma ceylanicum, and Ancyclostoma duodenale.

130. The method of claim 124, further comprising the step of chemically treating a hookworm-infected patient prior to said step of administering.

131. A nucleotide sequence represented by SEQ ID NO:

76.

132. An amino acid sequence represented by SEQ ID NO:

77.