Integrated support, integrated minute vessels, and permeable membrane, and method of making and using same
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Accused Products
Abstract
The present invention relates to an integrated support, integrated minute vessels and a permeable membrane, and a method of making and using the same, and has an object of providing an integrated support, integrated minute vessels and permeable membrane which can be manufactured simply, cheaply, and in large quantities; which can be suitably applied to various processes, and in which the level of integration is high, the operating efficiency is high, and wherein the volumes and sizes of the support, the vessels and the membrane are suitable for handling minute quantities of liquids; and for which processing can be conducted surely and with good reliability; as well as methods of making and using the same. The invention is constructed comprising at least one thread shaped, string shaped, tape shaped, or rod shaped long and slender base member, and a variety of substances for detection of predetermined chemical structure which are fixed side by side along the length of the base member, and the base member is rolled, laminated or arranged using a predetermined method, to give integration, and the fixed location on the layer surface of the integrated base member, of each substance for detection corresponds to the chemical structure thereof.
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Citations
49 Claims
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8. A DNA integrated support comprising:
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a substantially flat substrate wherein at least one thread shaped, string shaped, tape shaped, or rod shaped long and slender base member is rolled, laminated or arranged in such a way that either enables or prevents expansion while bringing side portions thereof into contact with each other or while maintaining a spacing or while sandwiching an auxiliary member, to give integration; and
genetic substances such as oligonucleotides which are fixed in locations along the length of said base member, either in a plurality of cavity sections which comprise channels, apertures or capillaries either with or without bottoms, or alternatively in a plurality of holding sections comprising a porous material, a foam material, a fibrous material, a material with an irregular surface, or an impregnating material, with the cavity sections or holding sections being provided along the length of said base member, and the fixed locations of each of said genetic substances correspond with base sequences for that substance.
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9. An integrated minute vessel comprising:
- at least one thread shaped, cord shaped, tape shaped or rod shaped long and slender base member; and
either cavity sections which comprise channels, apertures or capillaries with bottoms or ends, or holding sections having a porous material, a foam material, a fibrous material, a material with an irregular surface, or an impregnating material, provided on said base member, and wherein said base member is rolled, laminated or arranged to give integration. - View Dependent Claims (10, 11, 12, 13, 14, 15)
- at least one thread shaped, cord shaped, tape shaped or rod shaped long and slender base member; and
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16. A permeable membrane comprising:
- at least one thread shaped, string shaped, tape shaped, or rod shaped long and slender base member; and
cavity sections such as penetrating channels, apertures or capillaries, or holding sections formed from a porous material, a foam material, a fibrous material, a material with an irregular surface, or an impregnating material, provided on said base member, and wherein said base member is rolled, laminated or arranged to give integration. - View Dependent Claims (17, 18, 19, 20, 21)
- at least one thread shaped, string shaped, tape shaped, or rod shaped long and slender base member; and
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30. A method of manufacturing a DNA integrated support comprising:
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a positioning step in which at each of a plurality of predetermined locations positioned along the length of at least one thread shaped, cord shaped, tape shaped or rod shaped long and slender base member, a suspension or semiliquid incorporating a genetic substance such as an oligonucleotide with a predetermined base sequence which corresponds to that particular location is positioned and fixed onto said base member by being painted, dispensed, imprinted, drawn up, impregnated or stored, either into cavity sections provided on said base member comprising channels, apertures or capillaries, or into holding sections provided on said base member having a porous material, a foam material, a fibrous material, a material with an irregular surface, or an impregnating material; and
an integration step wherein said base member, on which is positioned the suspensions or semi-liquids, is rolled, laminated or arranged in such a way that either enables or prevents expansion, while bringing side portions of said base member into contact with each other or while maintaining a spacing or while sandwiching an auxiliary member to give integration.
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31. A method of manufacturing a DNA integrated support comprising:
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a positioning step in which at each of a plurality of predetermined locations side by side on at least one film type or thin sheet type base member, a suspension or semi-liquid incorporating a genetic substance such as an oligonucleotide with a predetermined base sequence which corresponds to that particular location is positioned and fixed onto said base member in substantially parallel lines, into cavity sections comprising channels, apertures or capillaries and which are provided in substantially parallel lines on said base member, or into thread shaped, cord shaped, tape shaped or rod shaped long and slender holding sections which are provided in substantially parallel lines on said base member and which have a porous material, a foam material, a fibrous material, a material with an irregular surface, or an impregnating material by being painted, dispensed, imprinted, drawn up, impregnated or stored;
an integration step wherein said base member, on which is positioned the suspensions or semi-liquids, is rolled, laminated or arranged in such a way that either enables or prevents expansion and so as not to bend said substantially parallel lines, while bringing face pairs of said base member into contact with each other or while maintaining a spacing or while sandwiching an auxiliary member, to give integration; and
a cutting step wherein said integrated base member is cut thinly across said substantially parallel lines to obtain a plurality of DNA integrated supports in which the cross-sectional surface of the cut functions as a layer surface.
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32. A method of manufacturing an integrated minute vessel comprising:
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a processing step for providing on at least one base member, either a plurality of cavity sections such as channels with bottoms or ends, or holding sections having a porous material, a foam material, a fibrous material, a material with an irregular surface, or an impregnating material; and
an integration step wherein said base member is rolled, laminated or arranged in such a way that either enables or prevents expansion, while bringing said base member into contact with itself or while maintaining a spacing or while sandwiching an auxiliary member, to give integration. - View Dependent Claims (33)
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34. A method of manufacturing a permeable membrane comprising:
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a processing step for providing on at least one base member, either a plurality of cavity sections such as bottomless or open-ended channels, or holding sections having a porous material, a foam material, a fibrous material, a material with an irregular surface, or an impregnating material; and
an integration step wherein said base member is rolled, laminated or arranged in such a way that either enables or prevents expansion, while bringing said base member into contact with itself or while maintaining a spacing or while sandwiching an auxiliary member to give integration. - View Dependent Claims (35)
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39. A method of using an integrated minute vessel comprising:
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a hardening step wherein with a base member of an integrated minute vessel in an expanded state, a predetermined suspension is positioned in a predetermined cavity section or a predetermined holding section by dispensing, imprinting, impregnating painting or storing, and subsequently hardened;
an integration step for integrating said base member on which said predetermined suspensions have been hardened, so that expansion is either enabled or prevented;
a fluidization step for fluidizing said hardened suspensions inside said respective cavity sections or holding sections; and
a processing step for conducting reaction processing within said cavity sections or holding sections. - View Dependent Claims (40, 41)
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- 42. An integrated medium storing fluid passage, comprising at least one fluid passage, and pressure control means for controlling the pressure inside said fluid passage, wherein an integrated support, an integrated minute vessel or a permeable membrane is stored inside said fluid passage, or inside a storage section communicated with said fluid passage, and said fluid is able to pass through the integrated support, integrated minute vessel or permeable membrane, or said fluid is able to contact the integrated support, integrated minute vessel or permeable membrane.
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49. A magnetic separation device comprising:
- a mounting section for mounting an integrated support, integrated minute vessel, or permeable membrane, and a magnetic section provided on a lower surface of said mounting section, and which enables the application of or removal of a magnetic field from beneath said substrate, with respect to the inside of each of said cavity sections of said integrated support, integrated minute vessel, or permeable membrane.
Specification