Binding phenol oxidizing enzyme-peptide complexes
First Claim
Patent Images
1. A binding peptide having the amino acid sequence illustrated in any one of SEQ ID NOS:
- 2 through 433.
1 Assignment
0 Petitions
Accused Products
Abstract
The present application relates to peptides which bind to a target stain, phenol oxidizing enzyme-binding peptide complexes wherein the binding peptide is attached to the C-terminus of the phenol oxidizing enzyme or is inserted or substituted into the phenol oxidizing enzyme. In a preferred embodiment the phenol oxidizing enzyme is a laccase specifically Stachybotrys oxidase B and variants thereof. The invention provides expression vectors comprising the phenol oxidizing enzyme-binding peptide complex as well as host cells comprising the vectors.
30 Citations
39 Claims
-
1. A binding peptide having the amino acid sequence illustrated in any one of SEQ ID NOS:
- 2 through 433.
- View Dependent Claims (2, 3, 4, 6, 26, 27, 28)
- 5. A binding peptide comprising a repeatable motif selected from the group consisting of SAPA, TAPP, APAL, PPP, PPPP, SSPH, SSP, SSK, SPT, LPAQ, PPPL, PTPL, SPTT, PLVP, PLP, YTKP, SLH, SLLNA, SPL, SNLA, SPLTQ, TTT, AARND, AARN, ARND, LSPG, NPNN, NLAT, NTS, PHSM, PPWM, PTSP, TGGA, YLPS, YTKP, PGSL, APS, TPV, TTTS and LNAT, wherein said binding peptide has 6 to 15 amino acid residues and binds to a stain on a fabric.
- 8. A phenol oxidizing enzyme-peptide complex comprising a phenol oxidizing enzyme and a peptide having the amino acid sequence illustrated in any one of SEQ ID NOS:
-
15. A laccase-peptide complex comprising a laccase obtainable from a Stachybotrys species and a peptide having an amino acid sequence illustrated in any one of SEQ ID NOS:
- 2 through 433.
- View Dependent Claims (16, 17, 18, 19, 20, 21)
-
25. A laccase-peptide complex comprising a peptide having the amino acid sequence illustrated in any one of SEQ ID NOs:
- 2-433 and a laccase, wherein said laccase comprises the amino acid sequence illustrated in SEQ ID NO;
1 or a variant thereof, wherein said variant differs in at least one of the positions 188, 254, 272, 346, 348, 394 and 425 of SEQ ID NO;
1.
- 2-433 and a laccase, wherein said laccase comprises the amino acid sequence illustrated in SEQ ID NO;
-
34. A method for producing a host cell comprising a polynucleotide encoding a laccase-peptide complex, comprising the steps of:
-
(a) obtaining a polynucleotide encoding a laccase having at least 68% identity to the amino acid sequence disclosed in SEQ ID NO;
1;
(b) obtaining a polynucleotide encoding a binding peptide having an amino acid sequence as illustrated in any one SEQ ID NOS;
2-433;
(c) conjugating the polynucleotide of step (a) with (b);
(d) introducing said conjugated polynucleotide into the host cell; and
(e) growing said host cell under conditions suitable for the production of said laccase-peptide complex. - View Dependent Claims (35, 36)
-
-
37. A method of using a binding peptide to target a stain on a textile comprising
a) obtaining a binding peptide as illustrated in any one of SEQ ID NOS: - 2-433;
b) exposing said binding peptide to a target stain, wherein said binding peptide binds to said stain and not to said textile. - View Dependent Claims (38)
- 2-433;
-
39. A method of enhancing the selectivity of a phenol oxidizing enzyme to a target stain which comprises,
a) derivatizing a laccase with a binding peptide as illustrated in any one of SEQ ID NOS: - 2-433 to form a laccase-peptide complex; and
b) exposing the laccase-peptide complex to a target stain, wherein selectivity of the laccase-peptide complex to the target stain is greater than the selectivity of a nonderivatized laccase having the same amino acid sequence as the laccase of the laccase-peptide complex.
- 2-433 to form a laccase-peptide complex; and
Specification