Microscopic image capturing apparatus, microscopic image capturing method, and storage medium recording microscopic image capturing program
First Claim
1. A microscopic image capturing apparatus comprising a microscope obtaining a light image with a sample illuminated by a light source, which exists within a viewing field, and a light image without the sample removed from the viewing field, which is captured in a state of being only illuminated by the light source, with an objective lens arranged as opposed to the sample, and a digital camera which is attached to the microscope and captures the light images obtained by the microscope, the digital camera comprising an A/D converting unit A/D-converting an image signal of each of the captured light images, a shading correction processing unit executing a shading correction process for digital image data converted by the A/D converting unit, a correction data storing unit storing shading correction data used when the shading correction process is executed by the shading correction processing unit, and a controlling unit, wherein:
- the correction data storing unit stores first shading correction data obtained based on image data of the light image without the sample, which is acquired by capturing the light image without the sample with the image capturing unit, in correspondence with a selection of non-removing a defective portion, and second shading correction data obtained based on an image acquired by using a median filter for the image data of the light image without the sample, which is obtained by capturing the light image without the sample with the image capturing unit, in correspondence with a selection of removing a defective portion; and
the controlling unit sets selected shading correction data in the shading correction processing unit from the correction data storing unit in correspondence with an externally input selection of using one of the first and the second shading correction data, when the shading correction process is executed for a image data of the light image with the sample obtained by capturing the light image with the sample with the image capturing unit.
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Accused Products
Abstract
In a correction data storing unit of a digital camera which captures a microscopic image, image data of a light image without a sample, image data obtained by using a filter for removing a defective portion for the light image without a sample, image data where a region without being corrected (or a region being corrected) is set in the light image without the sample, and shading correction data calculated from image data obtained by setting a mask region where data after being corrected is set to 0 are stored. An operator obtains, captures, records, and stores an observation image satisfactory for great-looking by using arbitrary shading correction data. In this way, a captured image satisfactory for great-looking is obtained by making a suitable shading correction according to a shading characteristic, which is caused by an optical system.
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Citations
33 Claims
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1. A microscopic image capturing apparatus comprising a microscope obtaining a light image with a sample illuminated by a light source, which exists within a viewing field, and a light image without the sample removed from the viewing field, which is captured in a state of being only illuminated by the light source, with an objective lens arranged as opposed to the sample, and a digital camera which is attached to the microscope and captures the light images obtained by the microscope, the digital camera comprising an A/D converting unit A/D-converting an image signal of each of the captured light images, a shading correction processing unit executing a shading correction process for digital image data converted by the A/D converting unit, a correction data storing unit storing shading correction data used when the shading correction process is executed by the shading correction processing unit, and a controlling unit, wherein:
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the correction data storing unit stores first shading correction data obtained based on image data of the light image without the sample, which is acquired by capturing the light image without the sample with the image capturing unit, in correspondence with a selection of non-removing a defective portion, and second shading correction data obtained based on an image acquired by using a median filter for the image data of the light image without the sample, which is obtained by capturing the light image without the sample with the image capturing unit, in correspondence with a selection of removing a defective portion; and
the controlling unit sets selected shading correction data in the shading correction processing unit from the correction data storing unit in correspondence with an externally input selection of using one of the first and the second shading correction data, when the shading correction process is executed for a image data of the light image with the sample obtained by capturing the light image with the sample with the image capturing unit. - View Dependent Claims (2, 3)
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4. A microscopic image capturing apparatus comprising a microscope obtaining a light image with a sample illuminated by a light source, which exists within a viewing field, and a light image without the sample removed from the viewing field, which is captured in a state of being only illuminated by the light source, with an objective lens arranged as opposed to the sample, and a digital camera which is attached to the microscope and captures the light images obtained by the microscope, the digital camera comprising an A/D converting unit A/D-converting an image signal of each of the captured light images, a shading correction processing unit executing a shading correction process for digital image data converted by the A/D converting unit, a correction data storing unit storing shading correction data used when the shading correction process is executed by the shading correction processing unit, and a controlling unit, wherein
the correction data storing unit stores shading correction data obtained by making a calculation based on image data of the light image without the sample, which is acquired by capturing the light image without the sample with the image capturing unit, and a restriction value set for the shading correction data.
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9. A microscopic image capturing apparatus comprising a microscope obtaining a light image with a sample illuminated by a light source, which exists within a viewing field, and a light image without the sample removed from the viewing field, which is captured in a state of being only illuminated by the light source, with an objective lens arranged as opposed to the sample, and a digital camera which is attached to the microscope and captures the light images obtained by the microscope, the digital camera comprising an A/D converting unit A/D-converting an image signal of each of the captured light images, a shading correction processing unit executing a shading correction process for digital image data converted by the A/D converting unit, a correction data storing unit storing shading correction data used when the shading correction process is executed by the shading correction processing unit, and a controlling unit, wherein
the controlling unit makes the correction data storing unit store data, which is obtained by using an average value filter for shading correction data, as final shading correction data, after the shading correction data is created from image data of the light image without the sample obtained by capturing the light image without the sample with the image capturing unit, and correction data except for a specified region in the shading correction data is replaced with 1, when the region to which the shading correction is to be made is specified in the image obtained by capturing the light image without the sample with the image capturing unit.
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10. A microscopic image capturing apparatus comprising a microscope obtaining a light image with a sample illuminated by a light source, which exists within a viewing field, and a light image without the sample removed from the viewing field, which is captured in a state of being only illuminated by the light source, with an objective lens arranged as opposed to the sample, and a digital camera which is attached to the microscope and captures the light images obtained by the microscope, the digital camera comprising an A/D converting unit A/D-converting an image signal of each of the captured light images, a shading correction processing unit executing a shading correction process for digital image data converted by the A/D converting unit, a correction data storing unit storing shading correction data used when the shading correction process is executed by the shading correction processing unit, and a controlling unit, wherein
the controlling unit separates defective portion component data from the shading correction data, and corrects the separated defective portion component data with a gain different from a gain of remaining component data to be shaded.
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12. A microscopic image capturing method in a digital camera, which is attached to a microscope, capturing a light image with a sample where the light image of the sample is within a viewing field, and light image without the sample, from which the sample is removed and which is only illuminated by the light source, within the viewing field of an objective lens, the light images being obtained by the microscope with the objective lens arranged as opposed to the sample illuminated by the light source, generating digital image data by A/D (analog/digital) converting an image signal of each of the captured light images, and executing a shading correction process by using shading correction data prestored in a correction data storing device for the digital image data of the light image with the sample, comprising:
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storing, in the correction data storing device, first shading correction data obtained based on image data of the light image without the sample, which is acquired by capturing the light image without the sample with the digital camera, in correspondence with a selection of non-removing a defective portion, and second shading correction data obtained based on an image, which is acquired by using a median filter for the image data of the light image without the sample acquired by capturing the light image without the sample with the digital camera, in correspondence with a selection of removing a defective portion; and
reading from the shading correction data storing device the first or the second shading data, which is selected according to an externally input selection of using one of the first and the second shading correction data in the shading correction process, and using the read first or second shading correction data in the shading correction process. - View Dependent Claims (13, 14)
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15. A microscopic image capturing method in a digital camera, which is incorporated in an image capturing unit attached to a microscope, capturing a light image with a sample where the light image of the sample is within a view of field, and light image without the sample, from which the sample is removed and which is only illuminated by the light source, within the view of field of an objective lens, the light images being obtained by the microscope with the objective lens arranged as opposed to the sample illuminated by the light source, generating digital image data by A/D (analog/digital) converting an image signal of each of the captured light images, and executing a shading correction process by using shading correction data prestored in a correction data storing device for digital image data of the light image with the sample, comprising
storing, in the correction data storing device, shading correction data obtained by making a calculation based on image data of the light image without the sample, which is obtained by capturing the light image without the sample with the digital camera, and a restriction value set for the shading correction data.
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20. A microscopic image capturing method in a digital camera, which is attached to a microscope, capturing a light image with a sample where the light image of the sample is within a viewing field, and light image without the sample, from which the sample is removed and which is only illuminated by the light source, within the viewing field of an objective lens, the light images being obtained by the microscope with the objective lens arranged as opposed to the sample illuminated by the light source, generating digital image data by A/D (analog/digital) converting an image signal of each of the captured light images, and executing a shading correction process by using shading correction data prestored in a correction data storing device for the digital image data of the light image with the sample, comprising
storing data, which is obtained by using an average value filter for shading correction data, in the correction data storing device as final shading correction data, after the shading correction data is created from the image data of the light image without the sample obtained by capturing the light image without the sample with the digital camera, and correction data except for a specified region in the shading correction data is replaced with 1, when the region to which the shading correction is to be made is specified in the image obtained by capturing the light image without the sample with the digital camera, in the shading correction process.
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21. A microscopic image capturing method in a digital camera, which is attached to a microscope, capturing a light image with a sample where the light image of the sample is within a viewing field, and light image without the sample, from which the sample is removed and which is only illuminated by the light source, within the viewing field of an objective lens, the light images being obtained by the microscope with the objective lens arranged as opposed to the sample illuminated by the light source, generating digital image data by A/D (analog/digital) converting an image signal of each of the captured light images, and executing a shading correction process by using shading correction data prestored in a correction data storing device for the digital image data of the light image with the sample, comprising
separating defective portion component data from the shading correction data, and correcting the separated defective portion component data with a gain different from a gain of remaining component data to be shaded, in the shading correction process.
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23. A computer-readable storage medium on which is recorded a program for causing a computer, which executes a microscopic image capturing method in a digital camera, which is attached to a microscope, capturing a light image with a sample where the light image of the sample is within a viewing field, and light image without the sample, from which the sample is removed and which is only illuminated by the light source, within the viewing field of an objective lens, the light images being obtained by the microscope with the objective lens arranged as opposed to the sample illuminated by the light source, generating digital image data by A/D (analog/digital) converting an image signal of each of the captured light images, and executing a shading correction process by using shading correction data prestored in a correction data storing device for the digital image data of the light image with the sample, to execute a process, the process comprising:
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storing, in the correction data storing device, first shading correction data obtained based on image data of the light image without the sample, which is acquired by capturing the light image without the sample with the image capturing unit, in correspondence with a selection of non-removing a defective portion, and second shading correction data obtained based on an image, which is acquired by using a median filter for the image data of the light image without the sample acquired by capturing the light image without the sample with the image capturing unit, in correspondence with a selection of removing a defective portion; and
reading from the shading correction data storing device the first or the second shading data, which is selected according to an externally input selection of using one of the first and the second shading correction data in the shading correction process, and using the read first or second shading correction data in the shading correction process. - View Dependent Claims (24, 25)
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26. A computer-readable storage medium on which is recorded a program for causing a computer, which executes a microscopic image capturing method in a digital camera, which is attached to a microscope, capturing a light image with a sample where the light image of the sample is within a viewing field, and light image without the sample, from which the sample is removed and which is only illuminated by the light source, within the viewing field of an objective lens, the light images being obtained by the microscope with the objective lens arranged as opposed to the sample illuminated by the light source, generating digital image data by A/D (analog/digital) converting an image signal of each of the captured light images, and executing a shading correction process by using shading correction data prestored in a correction data storing device for the digital image data of the light image with the sample, to execute a process, the process comprising
storing in the correction data storing device shading correction data obtained by making a calculation based on image data of the light image without the sample, which is obtained by capturing the light image without the sample with the digital camera, and a restriction value set for the shading correction data.
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31. A computer-readable storage medium on which is recorded a program for causing a computer, which executes a microscopic image capturing method in a digital camera, which is attached to a microscope, capturing a light image with a sample where the light image of the sample is within a viewing field, and light image without the sample, from which the sample is removed and which is only illuminated by the light source, within the viewing field of an objective lens, the light images being obtained by the microscope with the objective lens arranged as opposed to the sample illuminated by the light source, generating digital image data by A/D (analog/digital) converting an image signal of each of the captured light images, and executing a shading correction process by using shading correction data prestored in a correction data storing device for the digital image data of the light image with the sample, to execute a process, the process comprising
storing data, which is obtained by using an average value filter for shading correction data, in the correction data storing device as final shading correction data, after the shading correction data is created from the image data of the light image without the sample obtained by capturing the light image without the sample with the digital camera, and correction data except for a specified region in the shading correction data is replaced with 1, when the region to which the shading correction is to be made is specified in the image obtained by capturing the light image without the sample with the digital camera, in the shading correction process.
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32. A computer-readable storage medium on which is recorded a program for causing a computer, which executes a microscopic image capturing method in a digital camera, which is attached to a microscope, capturing a light image with a sample where the light image of the sample is within a viewing field, and light image without the sample, from which the sample is removed and which is only illuminated by the light source, within the viewing field of an objective lens, the light images being obtained by the microscope with the objective lens arranged as opposed to the sample illuminated by the light source, generating digital image data by A/D (analog/digital) converting an image signal of each of the captured light images, and executing a shading correction process by using shading correction data prestored in a correction data storing device for the digital image data of the light image with the sample, to execute a process, the process comprising
separating defective portion component data from the shading correction data, and correcting the separated defective portion component data with a gain different from a gain of remaining component data to be shaded, in the shading correction process.
Specification