Device for holding a substance library carrier

• US 20050047973A1
• Filed: 04/08/2004
• Published: 03/03/2005
• Est. Priority Date: 10/09/2001
• Status: Abandoned Application

• Current Assignee: Clondiag Chip Technologies GmbH (Abbott Laboratories)
• Original Assignee: Clondiag Chip Technologies GmbH (Abbott Laboratories)
• Inventors: Zieren, Martin, Ermantraut, Eugen, Kaiser, Thomas, Schulz, Torsten, Ellinger, Thomas, Poser, Siegfried, Tuchscheerer, Jens, Ullrich, Thomas, Fischer, Joachim, Mobius, Klaus-Peter
• Application Number:
  US10/820,269
• Publication Number:
  US 20050047973A1
• Time in Patent Office: Days
• US Class Current: 506/33
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00531 essentially square
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/141 Preventing contamination, tampering
• CPC Class Codes: B01L 2200/147 Employing temperature sensors
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2300/1805 Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046

Abstract

Disclosed are devices for holding a substance library carrier, and which are useful in conducting reactions in which binding between complementary molecules is measured qualitatively or quantitatively. Also disclosed are methods for conducting such reactions in the device.

Claims

1. A device for holding a substance library carrier, comprising two holding elements that are fixable with each other, and that form a layer composite comprising:

(i) a lid element having a detection surface with a substance library on its underneath side and being optically translucent at least in an area of the detection surface,

(ii) a sealing intermediate element having an enclosed recess; and

(iii) a base element being optically translucent at least in an area of the detection surface of the lid element,

wherein the lid element, the intermediate element and the base element together form an optically translucent chamber having a chamber space.

2. A device according to claim 1, wherein the base element comprises an integrated heating-temperature sensor device.

3. A device according to claim 1, wherein the base element comprises Borofloat 33, silica glass, monocrystalline CaF₂ and/or monocrystalline silicon.

4. A device according to claim 1, wherein the lid element comprises glass, Borofloat 33, quartz glass, monocrystalline CaF₂, monocrystalline silicon, phenylmethylmethacrylate and/or polycarbonate.

5. A device according to claim 1, wherein the intermediate element is elastic and can be repeatedly punctured from the side by cannulae, and that the chamber space remains sealingly closed upon extraction of the cannulae.

6. A device according to claim 1, wherein the intermediate element comprises polydimethyl siloxane, natural rubber, butadiene rubber, chloroprene rubber, nitrile butadiene rubber, butyl rubber, isoprene-styrene rubber, polynorbornene rubber, ethylene-proplyene rubber, fluor rubber, perfluor rubber, methyl-phenyl-silicon rubber, methyl-vinyl-silicon rubber, methyl-fluor-silicon rubber, fluor-silicon rubber, polysulfid rubber, urethane rubber, polyester or polyether prepolymers on the basis of 4,4′

-methylenedi(phenylisothiocyanate) or toluenediisocyanate.

7. A device according to claim 1, wherein the recess defines a geometrical form of the chamber space.

8. A device according to claim 1, wherein the chamber space may be filled free of air bubbles.

9. A device according to claim 1, wherein the chamber space is formed in the shape of a D, a new moon, or a sickle.

10. A device according to claim 1, wherein the chamber may be cooled.

11. A device according to claim 1, wherein the two holding elements are half shells engaging with one another and which are held together by press-fit when pressed together.

12. A device according to claim 1, wherein the holding elements each comprise channels for cooling the chamber.

13. A device according to claim 1, wherein the holding elements each comprise a recess for receiving a slide or lug for loading the sample chamber.

14. A device according to claim 13, further comprising a media connection for heating the chamber, and a media connection for cooling the chamber, and a recess for receiving an injection apparatus, and wherein the media connections and the recesses are located on one side of the device.

15. A device according to claim 1, which is attached to a connector.

16. A device according to claim 15, which may be operated fully automatically through the connector.

17. A device according to claim 1, which is attached to a manual filling station.

18. A device according to claim 1, which contains a protein library.

19. A device according to claim 18, wherein the protein library is an antibody library, a receptor protein library or a membrane protein library.

20. A device according to claim 1, which contains a peptide library.

21. A device according to claim 20, wherein the peptide library is a receptor ligand library, a library of pharmacologically active peptides or a library of peptide hormones.

22. A device according to claim 1, which contains a nucleic acid library.

23. A device according to claim 22, wherein the nucleic acid library is a DNA molecule library.

24. A device according to claim 22, wherein the nucleic acid library is an RNA molecule library.

25. A method of carrying out a microarray-based test, comprising (a) providing a device according to claim 1, wherein the substance library contains a microarray having fixed thereon probes that bind a target molecule;

(b) introducing a sample suspected of containing the target molecule into the device; and

(c) detecting presence or amount of interaction between the probes and the target molecule.

26. A method according to claim 25, wherein the substance library contains a protein library.

27. A method according to claim 26, wherein the protein library is an antibody library, a receptor protein library or a membrane protein library.

28. A method according to claim 26, wherein the sample contains nucleic acids.

29. A method according to claim 26, wherein the sample contains proteins.

30. A method according to claim 25, wherein the substance library contains a peptide library.

31. A method according to claim 28, wherein the peptide library is a receptor ligand library, a library of pharmacologically active peptides or a library of peptide hormones.

32. A method according to claim 25, wherein the substance library contains a nucleic acid library.

33. A method according to claim 32, wherein the sample contains nucleic acids.

34. A method according to claim 33, wherein the nucleic acids are obtained from an organism.

35. A method according to claim 33, wherein the nucleic acids are obtained from a cell.

36. A method according to claim 33, wherein the nucleic acids are obtained from a microorganism.

37. A method according to claim 36, wherein the microorganism is pathogenic.

38. A method according to claim 32, wherein the nucleic acid library is an RNA molecule library.

39. A method according to claim 32, wherein the nucleic acid library is an DNA molecule library.

40. A method according to claim 33, wherein said sample comprises a polymerase chain reaction (PCR) mixture comprising said nucleic acid, at least one primer, nucleotides and a polymerase, such that said nucleic acids also undergo amplification via PCR.

41. A method according to claim 40, wherein said PCR mixture comprises two primers, one of which is fluorescently labeled.

42. A method according to claim 33, wherein said nucleic acids also undergo a ligase chain reaction (LCR).

43. A method according to claim 33, wherein said nucleic acids also undergo a ligase detection reaction (LDR).

44. A first device for filling a second device for holding a substance library carrier, wherein the second device comprises two holding elements that are fixable with each other, and that form a layer composite comprising:

(i) a lid element having a detection surface with a substance library on its underneath side and being optically translucent at least in an area of the detection surface,

(ii) a sealing intermediate element having an enclosed recess; and

(iii) a base element being optically translucent at least in an area of the detection surface of the lid element,

wherein the lid element, the intermediate element and the base element together form an optically translucent sample chamber having a chamber space,

and wherein the first device comprises a body and a cover fixable to the body, wherein the body contains recesses for a filling unit, a ventilation unit and the second device, and wherein the recesses are arranged such that the sample chamber of the second device may be loaded and vented through puncturing of the intermediate element from its side.

45. A device according to claim 44, wherein the filling unit comprises a syringe with a first cannula, and wherein the ventilation unit comprises a second canula.