Methods for genetic analysis

• US 20050048531A1
• Filed: 03/25/2004
• Published: 03/03/2005
• Est. Priority Date: 09/17/1998
• Status: Abandoned Application

• Current Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Lockhart, David J., Mack, David H., Mittman, Michael
• Application Number:
  US10/809,189
• Publication Number:
  US 20050048531A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C07H 21/02 with ribosyl as saccharide radical
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

The invention provides nucleic acid sequences which are complementary, in one embodiment, to a wide variety of murine genes. The invention provides the sequences in such a way as to make them available for a variety of analyses. As such, the invention related to diverse fields impacted by the nature of molecular interaction, including chemistry, biology, medicine, and medical diagnostics.

Claims

1-76. -76. (cancelled)

77. An array comprising a plurality of nucleic acid probes wherein each probe in said plurality of nucleic acid probes consists essentially of one of the sequences listed in SEQ ID Nos. 1-127,811 and wherein said plurality of nucleic acid probes of the array comprises each of the sequences listed in SEQ ID Nos. 1-127,811.

78. An array comprising 127,811 different sequence nucleic acid probes attached to a solid support at known locations, wherein each different sequence nucleic acid probe is 20 to 30 nucleotides and is completely complementary to a region of the mouse genome.

79. A method of monitoring the expression of a plurality of mouse genes in a first nucleic acid sample obtained from a mouse sample, said method comprising:

obtaining a second nucleic acid sample by amplifying a plurality of nucleic acids in said first sample;

hybridizing said second nucleic acid sample to the array of claim 78 to obtain a hybridization pattern; and

analyzing said hybridization pattern to obtain a measurement of the expression of a plurality of mouse genes.