Chip for processing of gene and apparatus for processing of gene
First Claim
1. A chip for processing of gene that is equipped with an injection port into which a sample containing gene is delivered, a gene extraction part into which a solution containing said sample is introduced and which has a gene-binding carrier that binds to said gene, a washing solution-storing part that stores the washing solution to be introduced into said gene extraction part, and a reaction part into which said gene captured in said extraction part is introduced, wherein a fluid channel through which said washing solution is introduced from said washing solution-storing part has been connected to a region more remote from said injection port than from a region into which a solution containing said sample is introduced in said gene extraction part.
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Abstract
The present invention provides an analytical chip that is easy to handle, inexpensive, and for which the extraction of gene from a sample and analysis thereof can be automated to one process, and a small-sized and portable analytical apparatus equipped therewith. The chip for processing of gene that is equipped with an injection port into which a sample containing gene is delivered, a gene extraction part into which a solution containing said sample is introduced and which has a gene-binding carrier that binds to said gene, a washing solution-storing part that stores the washing solution to be introduced into said gene extraction part, and a reaction part into which said gene captured in said extraction part is introduced, wherein a fluid channel through which said washing solution is introduced from said washing solution-storing part has been connected to a region more remote from said injection port than from a region into which a solution containing said sample is introduced in said gene extraction part is obtained.
120 Citations
15 Claims
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1. A chip for processing of gene that is equipped with
an injection port into which a sample containing gene is delivered, a gene extraction part into which a solution containing said sample is introduced and which has a gene-binding carrier that binds to said gene, a washing solution-storing part that stores the washing solution to be introduced into said gene extraction part, and a reaction part into which said gene captured in said extraction part is introduced, wherein a fluid channel through which said washing solution is introduced from said washing solution-storing part has been connected to a region more remote from said injection port than from a region into which a solution containing said sample is introduced in said gene extraction part.
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2. A chip for processing of gene that is equipped with
an injection port into which a sample containing gene is delivered, a gene extraction part into which said sample is introduced and which has a gene-binding carrier that binds to said gene, a washing solution-storing part that stores the washing solution to be introduced into said gene extraction part, and a reaction part into which the gene extracted at said gene extraction part is introduced, wherein it has been formed so that said washing solution that has been introduced from said washing solution-storing part to said gene extraction part flows into said injection port end after flowing out of said gene extraction part.
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3. A chip for processing of gene that is equipped with
an injection port into which a sample containing gene is delivered, a gene extraction part into which said sample is introduced and which has a gene-binding carrier that captures said gene, a washing solution-storing part that stores the washing solution to be introduced into said gene extraction part, and a reaction part into which the gene extracted at said gene extraction part is introduced, wherein said injection port, said gene extraction part, and said washing solution-storing part have been arranged in series through a fluid channel.
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4. A chip for processing of gene that is equipped with
an injection port into which a sample containing gene is delivered, a dissolving solution-storing part that stores the dissolving solution to be introduced to said sample that has been delivered to said injection port, a gene extraction part into which a mixture of said sample and said dissolving solution is introduced and which has a gene-binding carrier which binds to said gene, a washing solution-storing part that stores the washing solution to be introduced into said gene extraction part, an eluting solution-storing part that stores the eluting solution to be introduced into said gene extraction part, and a reaction part into which said gene eluted from said eluting solution is introduced, and that has a fluid channel which branches from between said injection port and said gene extraction part and is connected to said reaction part.
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5. A chip for processing of gene that is equipped with
an injection port into which a sample containing gene is delivered, a dissolving solution-storing part that stores the dissolving solution to be introduced into the sample that has been delivered into said injection port, a gene extraction part into which a mixture of said sample and said dissolving solution is introduced and which has a gene-binding carrier that captures said gene, a washing solution-storing part that stores the washing solution to be introduced into said gene extraction part, and an eluting solution-storing part that stores the eluting solution to be introduced into said gene extraction part, wherein either storing part of said dissolving solution-storing part, said washing solution-storing part, and said eluting solution-storing part is formed by connecting, through bending parts, a plurality of fluid channels longer in length in the longitudinal direction than in width, and the other end of said storing part has an introducing part of a fluid to be introduced when said solution stored is discharged from the storing part.
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8. A chip for processing of gene equipped with:
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the first fluid introduction part that is equipped with an injection port into which a sample containing gene is delivered, a dissolving solution-storing part that stores the dissolving solution to be introduced into the sample that has been delivered into said injection port, a gene extraction part into which a mixture of said sample and said dissolving solution is introduced and which has a gene-binding carrier that captures said gene, a washing solution-storing part that stores the washing solution to be introduced into said gene extraction part, an eluting solution-storing part that stores the eluting solution to be introduced into said gene extraction part, and a reaction part into which said gene eluted with the eluting solution is introduced, and that is located at the side more remote from said injection port than from the region in which said dissolving solution has been stored in said dissolving solution-storing part and in which a fluid is delivered to said dissolving solution-storing part when said dissolving solution is introduced into said injection port, the first fluid discharge part that discharges the fluid in said gene extraction part out of said gene extraction part before said sample and said dissolving solution are introduced into a region more remote from said injection port than the area into which said sample and said dissolving solution are introduced in said gene extraction part, the second fluid introduction part into which a fluid is delivered when said washing solution is introduced into said injection port at the side more remote from said gene capturing part than the area in which said washing solution has been stored in said washing solution-storing part, the third fluid introduction part into which a fluid is delivered when said eluting solution is introduced into said injection port at the side more remote from said gene capturing part than the area in which said eluting solution of said eluting solution-storing part has been stored in said eluting solution-storing part, and the fourth fluid introduction part into which a fluid is delivered when said solution containing said eluted gene is introduced from said gene extraction part into said reaction part.
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9. An apparatus for processing of gene which is equipped with:
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a chip mounting part having mounted thereon a chip for processing of gene that has an injection port into which a sample containing gene is delivered, a gene extraction part into which a solution containing said sample is introduced and which is equipped with a gene-binding carrier that captures said gene, a washing solution-storing part that stores the washing solution to be introduced into said gene extraction part, and a reaction part into which said gene captured in said gene extraction part is introduced; and
a fluid introduction mechanism that introduces a fluid into said chip for processing of gene; and
a detection mechanism that detects the eluted gene, wherein, said apparatus is controlled so that the washing solution that was introduced from said washing solution-storing part to said gene extraction part flows from said gene extraction part to said injection port.
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10. An apparatus for processing of gene which is equipped with:
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a chip mounting part having mounted thereon a chip for processing of gene that has an injection port into which a sample containing gene is delivered, a gene extraction part, formed in connection with the injection port, into which a solution containing said sample is introduced and which is equipped with a gene extraction part having a gene-binding carrier that captures said gene, and a washing solution-storing part formed in connection with said gene extraction part, and that is equipped with a fluid connection part of the gene extraction part downstream of said gene extraction part relative to said injection port wherein the outside and the fluid are connected, and a fluid connection part of the washing solution-storing part downstream of said washing solution-storing part relative to said gene extraction part wherein the outside and the fluid are connected;
a fluid control mechanism that introduces or aspirates a fluid into said chip for processing of gene; and
a detection mechanism that detects the gene contained in said sample, wherein, said fluid connection part of the gene extraction part is controlled to permit the flow of a fluid between the inside of said chip and the outside of said chip, and said fluid connection part of the washing solution-storing part is controlled to limit the flow of a fluid between the inside of said chip and the outside of said chip, thereby to control to permit the introduction of a solution containing said sample from said injection port to said gene extraction part, and said fluid connection part of the gene extraction part is controlled to limit the flow of a fluid between the inside of said chip and the outside of said chip, and said fluid connection part of the washing solution-storing part is controlled to permit the flow of a fluid between the inside of said chip and the outside of said chip, thereby to control to permit the flow of said washing solution from said washing solution-storing part through said gene extraction part to said injection port. - View Dependent Claims (11, 12)
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13. A method of using a chip for processing of gene comprising the steps of:
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cooling and freezing a chip for processing of gene having a sample injection port into which a test sample is injected, a reagent tank, in connection with said sample injection port, in which reagents have been stored, a fluid channel for extracting gene from said test sample, a reactor in which the extracted gene is detected, and a fluid channel connecting said reagent tank and the external fluid channel, and carrying said frozen chip for processing of gene.
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14. A method of using a chip for processing of gene comprising the steps of:
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cooling and refrigeration a chip for processing of gene having a sample injection port into which a test sample is injected, a reagent tank, in connection with said sample injection port, in which reagents have been stored, a fluid channel for extracting gene from said test sample, a reactor in which the extracted gene is detected, and a fluid channel connecting said reagent tank and the external fluid channel, and carrying said frozen chip for processing of gene.
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15. A method of detecting gene comprising the steps of:
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providing a chip for processing of gene having a sample injection port for injecting a test sample containing a gene, a reagent tank, connected to said sample injection port, in which reagents have been stored, a fluid channel for extracting gene from the test sample, a reactor for detecting the extracted gene, and a fluid channel connecting said reagent tank and the external fluid channel after the chip was once cooled, refrigerated or frozen;
bringing the provided analytical chip back to room temperature;
introducing a sample containing the gene into said sample injection port and extracting gene from said reagent with said reagent; and
detecting said gene.
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Specification