Porous glass substrates with reduced auto-fluorescence
First Claim
1. A porous substrate comprising:
- a support; and
a porous region on a surface of said support, the porous region being of primarily inorganic material and having a surface upon which a number of probe molecules can be immobilized, the porous region having a tint and exhibits a reduced level of auto-fluorescence of at least about 15% relative to a comparable non-tinted porous substrate surface.
1 Assignment
0 Petitions
Accused Products
Abstract
A porous inorganic substrate that may be used as a support, upon which biological, biochemical, or chemical reactions may be executed, is provided. A porous layer of the substrate is tinted with a colorant agent, and the porous substrate has at least about 15-20% less auto-fluorescence background relative to a conventional “white” porous substrate. The tinted porous layer provides improved signal to noise ratios that are important in detection metrics when performing biological or chemical binding assays. Once the porous layer is functionalized, one may immobilize probe molecules on or within the porous layer to create a microarray having a greater probe concentration and retention capability than conventional non-porous inorganic substrates, without suffering relatively high levels of auto-fluorescence and other detriments common to un-tinted porous substrates.
-
Citations
23 Claims
-
1. A porous substrate comprising:
- a support; and
a porous region on a surface of said support, the porous region being of primarily inorganic material and having a surface upon which a number of probe molecules can be immobilized, the porous region having a tint and exhibits a reduced level of auto-fluorescence of at least about 15% relative to a comparable non-tinted porous substrate surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
- a support; and
-
18. A tool for performing biological or chemical assays, the tool comprises a non-porous support;
- and a porous region on a surface of said support, the porous region being of primarily inorganic material and having a surface upon which a number of probe molecules may be immobilized, the porous region having a tint and exhibits a reduced level of auto-fluorescence of at least about 15% relative to a comparable non-tinted porous substrate surface.
- View Dependent Claims (19, 20, 21, 22, 23)
Specification