Methods of screening for monoclonal antibodies with desirable activity
First Claim
1. A method for producing a library of monoclonal antibodies, said method comprising:
- a) altering a nucleic acid encoding a selected humanized monoclonal antibody to provide a library of humanized monoclonal antibody-encoding nucleic acids; and
b) introducing said library into mammalian cells to produce monoclonal antibodies on the surfaces of said cells to obtain said library of monoclonal antibodies.
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Abstract
The invention provides methods for screening for a monoclonal antibody with a desirable activity. The methods involve altering a nucleic acid encoding a selected parental humanized monoclonal antibody to make a library of nucleic acids, introducing the library into mammalian cells such that a library of monoclonal antibodies are produced on the surfaces of the mammalian cells, and sorting the cells to isolate a cell producing a humanized monoclonal antibody with a desirable activity, e.g., increased affinity for an binding partner as compared to the parental antibody. This isolated cell may be cultured and used to reiterate the method. The subject methods find use in a variety of different industrial, medical and research applications.
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20 Claims
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1. A method for producing a library of monoclonal antibodies, said method comprising:
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a) altering a nucleic acid encoding a selected humanized monoclonal antibody to provide a library of humanized monoclonal antibody-encoding nucleic acids; and
b) introducing said library into mammalian cells to produce monoclonal antibodies on the surfaces of said cells to obtain said library of monoclonal antibodies. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification