Electromagnetic fields increase in vitro and in vivo angiogenesis through endothelial release of FGF-2

• US 20050049640A1
• Filed: 05/11/2004
• Published: 03/03/2005
• Est. Priority Date: 05/12/2003
• Status: Abandoned Application

• Current Assignee: New York University, EBI LP (Zimvie Incorporated)
• Original Assignee: New York University
• Inventors: Levine, Jamie, Tepper, Oren, Gurtner, Geoffrey C.
• Application Number:
  US10/843,141
• Publication Number:
  US 20050049640A1
• Time in Patent Office: Days
• US Class Current: 607/2
• CPC Class Codes: A61N 2/00 Magnetotherapy
• CPC Class Codes: A61N 2/02 using magnetic fields produced by coils, including single turn loops or electromagnets A61N2/12 takes precedence

Abstract

The present invention relates to a method of inducing angiogenesis in a cell or tissue by applying an electromagnetic field to the cell or tissue under conditions effective to induce angiogenesis. Also disclosed is a method of treating an ischemic condition in a patient by applying an electromagnetic field to ischemic tissue in a patient under conditions effective to treat the ischemic condition by inducing angiogenesis. A method of tissue engineering is also disclosed. This method involves providing a tissue scaffold and subjecting the tissue scaffold to an electromagnetic field under conditions effective to form a vascularized tissue scaffold. Further disclosed is a method of inducing activity of angiogenic growth factors by applying an electromagnetic field to a cell or tissue under conditions effective to induce activity of an angiogenic growth factor.

Claims

1. A method of inducing angiogenesis in a cell or tissue, said method comprising:

applying an electromagnetic field to the cell or tissue under conditions effective to induce angiogenesis.

2. The method according to claim 1, wherein the cell is an endothelial cell or the tissue comprises endothelial cells.

3. The method according to claim 1, wherein the electromagnetic field comprises pulsed electromagnetic fields.

4. The method according to claim 1, wherein said applying an electromagnetic field is carried out with a device capable of emitting an electromagnetic field.

5. The method according to claim 4, wherein the device capable of emitting an electromagnetic field is a pulsed electromagnetic field device which operates at a frequency of about 5 to 25 Hz.

6. The method according to claim 4, wherein the device capable of emitting an electromagnetic field is a pulsed electromagnetic field device which produces asymmetric pulses lasting for about 1 to 10 msec.

7. A method of treating an ischemic condition in a patient, said method comprising:

applying an electromagnetic field to ischemic tissue in a patient under conditions effective to treat the ischemic condition by inducing angiogenesis.

8. The method according to claim 7, wherein the electromagnetic field comprises pulsed electromagnetic fields.

9. The method according to claim 7, wherein the ischemic condition is coronary artery disease.

10. The method according to claim 7, wherein the ischemic condition is peripheral vascular disease.

11. The method according to claim 7, wherein the ischemic condition is cerebrovascular disease.

12. The method according to claim 7, wherein the ischemic condition is a wound.

13. The method according to claim 12, wherein the wound is a chronic wound.

14. The method according to claim 12, wherein the wound is a diabetic wound.

15. The method according to claim 7, wherein the electromagnetic field is applied to surgically exposed tissue.

16. The method according to claim 7, wherein the electromagnetic field is applied to skin overlying tissue.

17. The method according to claim 7, wherein the ischemic tissue is positioned within an electromagnetic field device.

18. The method according to claim 7, wherein said applying comprises:

providing a device capable of emitting an electromagnetic field, and subjecting the ischemic tissue to an electromagnetic field with said device.

19. The method according to claim 18, wherein the device capable of emitting an electromagnetic field is a pulsed electromagnetic field device which operates at a frequency of about 5 to 25 Hz.

20. The method according to claim 18, wherein the device capable of emitting an electromagnetic field is a pulsed electromagnetic field device which produces asymmetric pulses lasting for about 1 to 10 msec.

21. The method according to claim 7, wherein the patient is human.

22. A method of tissue engineering comprising:

providing a tissue scaffold, and subjecting the tissue scaffold to an electromagnetic field under conditions effective to form a vascularized tissue scaffold.

23. The method according to claim 22, wherein the electromagnetic field comprises pulsed electromagnetic fields.

24. The method according to claim 22, wherein said subjecting is carried out in vivo.

25. A method of inducing activity of angiogenic growth factors, said method comprising:

applying an electromagnetic field to a cell or tissue under conditions effective to induce activity of an angiogenic growth factor.

26. The method according to claim 25, wherein the electromagnetic field comprises pulsed electromagnetic fields.

27. The method according to claim 25, wherein said applying comprises:

applying the electromagnetic field with a device capable of emitting an electromagnetic field.

28. The method according to claim 27, wherein the device capable of emitting an electromagnetic field is a pulsed electromagnetic field device which operates at a frequency of about 5 to 25 Hz.

29. The method according to claim 27, wherein the device capable of emitting an electromagnetic field is a pulsed electromagnetic field device which produces asymmetric pulses lasting for about 1 to 10 msec.

30. The method according to claim 25, wherein the angiogenic growth factor is Fibroblast Growth Factor 2.