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Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents

  • US 20050053939A1
  • Filed: 11/08/2002
  • Published: 03/10/2005
  • Est. Priority Date: 11/09/2001
  • Status: Abandoned Application
First Claim
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1. A method for isolating a signal produced by a detectable moiety of a released predetermined portion of a probe from signal obtained from intact probe and from other fragments thereof, the method comprising:

  • (a) combining a probe which comprises a first predetermined portion which is releasable and includes a detectable moiety, and a second portion which includes at least one interactive functionality, with a reagent that potentiates the release of the first portion from the probe;

    (b) subjecting the resulting mixture to conditions under which the probe is cleaved, such that the first portion is released from the probe;

    (c) contacting the mixture with a capture reagent which binds the interactive functionality, and (d) separating the capture agent or the released predetermined first portion from the mixture.

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