Biochip for proteomics applications

• US 20050053949A1
• Filed: 09/08/2003
• Published: 03/10/2005
• Est. Priority Date: 09/08/2003
• Status: Abandoned Application

• Current Assignee: Vitalii Ivanovich Silin
• Original Assignee: Vitalii Ivanovich Silin
• Inventors: Silin, Vitalii Ivanovich
• Application Number:
  US10/656,369
• Publication Number:
  US 20050053949A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00432 Photolithographic masks
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00729 Peptide nucleic acids [PNA]
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 33/54386 : Analytical elements

Abstract

Arrays of binding molecules/instigators useful for the simultaneous detection of a plurality of proteins, which are the expression products, or fragments thereof, of a cell, or population of cells in an organism are provided. Methods of both making and using the arrays are also disclosed. The arrays are particularly useful for various proteomics applications including assessing patterns of protein expression and modification in cells and can be useful for biowarfare agents detection.

Claims

1. An array device comprising:

a substrate having a surface;

one or more binding molecules on said substrate surface; and

one or more instigators, said one or more instigators each comprising (a) a single stranded DNA or RNA or PNA compliment to one or more of said binding molecules and (b) a protein-binding moiety, said protein-binding moiety is specific for a protein.

2. The device of claim 1 wherein at least two of said binding molecules are adjacent each other.

3. The device of claim 2 wherein at least two of said binding molecules are separated from each other by one or more border regions.

4. The device of claim 3 wherein said border regions are formed from non-binding molecules that prevent non-specific adsorption.

5. The device of claim 3 wherein said non-binding molecules are hydrophobic.

6. The device of claim 3 wherein said non-binding molecules are hydrophilic.