Amplification of nucleic acids with electronic detection
First Claim
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1. A method for detecting the presence of a ribosomal RNA target sequence in a nucleic acid sample, comprising:
- a) applying an electronic input signal to a surface comprising an array of electrodes wherein said electrodes comprise a hybridization complex and said hybridization complex comprises;
i) a capture probe;
ii) an electron transfer moiety; and
ii) a ribosomal RNA target nucleic acid;
b) detecting the presence of said ribosomal RNA target sequence via an output signal.
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Abstract
The invention relates to compositions and methods useful in the detection of nucleic acids using a variety of amplification techniques, including both signal amplification and target amplification. Detection proceeds through the use of an electron transfer moiety (ETM) that is associated with the nucleic acid, either directly or indirectly, to allow electronic detection of the ETM using an electrode.
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Citations
26 Claims
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1. A method for detecting the presence of a ribosomal RNA target sequence in a nucleic acid sample, comprising:
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a) applying an electronic input signal to a surface comprising an array of electrodes wherein said electrodes comprise a hybridization complex and said hybridization complex comprises;
i) a capture probe;
ii) an electron transfer moiety; and
ii) a ribosomal RNA target nucleic acid;
b) detecting the presence of said ribosomal RNA target sequence via an output signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13)
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14. A method for detecting the presence of a ribosomal RNA target sequence in a nucleic acid sample, comprising:
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a) applying an electronic input signal to a surface comprising an array of electrodes wherein said electrodes comprise a hybridization complex and said hybridization complex comprises;
i) a capture binding ligand;
ii) a solution binding ligand;
iii) an electron transfer moiety; and
iv) a ribosomal RNA target nucleic acid;
b) detecting the presence of said ribosomal RNA target sequence via an output signal. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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Specification