Methods and compositions for whole genome amplification and genotyping
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Abstract
This invention provides methods of amplifying genomic DNA to obtain an amplified representative population of genome fragments. Methods are further provided for obtaining amplified genomic DNA representations of a desired complexity. The invention further provides methods for simultaneously detecting large numbers of typable loci for an amplified representative population of genome fragments. Accordingly the methods can be used to genotype individuals on a genome-wide scale.
71 Citations
104 Claims
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1-77. -77. (cancelled).
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78. A method, comprising
(a) contacting a plurality of genome fragments with a plurality of different immobilized nucleic acid probes under conditions wherein immobilized probe-fragment hybrids are formed; -
(b) modifying said immobilized probes while hybridized to said genome fragments, thereby forming modified immobilized probes;
(c) removing said genome fragments from said probe-fragment hybrids; and
(d) detecting said modified immobilized probes after removing said genome fragments, thereby detecting typable loci of said genome fragments. - View Dependent Claims (79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104)
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Specification