Automated biological growth and dispensing system

• US 20050054086A1
• Filed: 08/30/2004
• Published: 03/10/2005
• Est. Priority Date: 09/04/2003
• Status: Abandoned Application

• Current Assignee: Gotohti.Com Inc.
• Original Assignee: Gotohti.Com Inc.
• Inventors: Ophardt, Heiner
• Application Number:
  US10/928,109
• Publication Number:
  US 20050054086A1
• Time in Patent Office: Days
• US Class Current: 435/296.100
• CPC Class Codes: C12M 23/00 Constructional details, e.g. recesses, hinges flow directing inserts in C12M27/18-C12M27/24; apparatus for chemical or physical processes in general B01J, chemical or physical laboratory apparatus in general B01L
• CPC Class Codes: C12M 23/28 disposable or single use
• CPC Class Codes: C12M 23/38 Caps; Covers; Plugs; Pouring means
• CPC Class Codes: C12M 23/40 Manifolds; Distribution pieces fluid transfer means B01L3/563
• CPC Class Codes: C12M 23/44 Multiple separable units; Modules
• CPC Class Codes: C12M 27/02 Stirrer or mobile mixing elements
• CPC Class Codes: Y02W 10/37 using solar energy

Abstract

An automated biological growth and dispensing system utilizing a modular growing tank which is removable for replacement by another growing tank. Mechanisms for the delivery of air, water and/or nutrients are adapted to permit the growing tank to be readily coupled and uncoupled for easy and inexpensive replacement. Mechanisms for agitation may be integral and removable with the growing tank or may be adapted for a quick connection and disconnection with the growing tank. The growing tank may be disposable and each new growing tank may be provided as a sealed container including a starting amount of a biomass and/or nutrient.

Claims

1. An automated batch apparatus for growing bacteria comprising:

a modular bio-generation tank having a top, a bottom, a water inlet for entry of water into the tank, an air inlet for entry of air into the tank, a nutrient inlet for entry of nutrients into the tank, and a tank outlet for flow of fluid from the tank,

an agitation mechanism for agitating fluid in the tank,

an air delivery system for delivery of air via the air inlet into the tank into contact with fluid in the tank,

a water delivery system for delivery of water via the water inlet into the tank,

a nutrient delivery system for delivery of nutrient via the nutrient inlet into the tank, the tank being removable.

2. The apparatus according to claim 1, characterized in that it includes a delivery manifold removably coupled to the tank via a quick connect and disconnect arrangement to deliver water, air and nutrients to the tank,

the manifold connected to the water delivery system to receive water, and deliver water to the water inlet of the tank when the manifold and tank are coupled,

the manifold connected to the air delivery system to receive air and deliver air to the air inlet of the tank when the manifold and tank are coupled,

the manifold connected to the nutrient delivery system to receive nutrients and deliver nutrients to the nutrient inlet of the tank when the manifold and tank are coupled, wherein by coupling the tank to the manifold, the tank becomes operatively coupled to the water delivery system, the air delivery system and the nutrient delivery system.

3. The apparatus according to claim 2, characterized in that the manifold comprises a removable top lid for the tank.

4. The apparatus according to claim 3, characterized in that the water inlet, air inlet and nutrient inlet are provided at the top of the tank.

5. The apparatus according to claim 1, characterized in that the top of the tank is open upwardly providing a tank inlet opening which comprises the water inlet, air inlet and the nutrient inlet, the manifold comprises a removable top lid for the tank covering the inlet opening.

6. The apparatus according to claim 5, characterized in that the agitation mechanism comprises an impeller in the tank for rotation to agitate fluid therein.

7. The apparatus according to claim 6, characterized in that the agitation mechanism further comprises a motor external of the tank coupled to the impeller to rotate the impeller.

8. The apparatus according to claim 7, characterized in that the motor and impeller are coupled to the tank to be removable and replaceable with the tank, a power supply to the motor being removably coupled to the motor by a quick connect and disconnect arrangement.

9. The apparatus according to claim 8, characterized in that the motor comprises an electric motor secured to the bottom of the tank external of the tank, the motor coupled to the impeller by a shaft extending in fluid sealed relation through the bottom of the tank to the impeller within the tank.

10. The apparatus according to claim 9, characterized in that the tank including the motor and impeller are a modular disposable unit.

11. The apparatus according to claim 1, characterized in that a plurality of said modular bio-generation tanks are provided with a different tank to be used in each batch,

each tank carries within the interior of the tank for shipment and storage prior to use in a batch with starter materials selected from one or more of a batch starter population of bacteria, nutrients and water,

a closure removably coupled to the tank for retaining the starter materials therein prior to use in a batch.

12. The apparatus according to claim 11, characterized in that one or more of the starter materials is provided within the tank in a separate container to keep starter materials confined or separated from other starter materials.

13. The apparatus according to claim 1, characterized in that it includes a plurality of the modular bio-generation tanks coupled together in parallel for simultaneous growth of bacteria therein, each tank being substantially the same and replaceable by other similar modular tanks after each batch.

14. The apparatus according to claim 2, characterized in that the air delivery system comprises an air fan to blow air via the air inlet opening into the tank and into contact with fluid in the tank.

15. The apparatus according to claim 5, characterized in that the air delivery system comprises an electrically powered air fan carried by the top lid to blow air through an air opening in the lid through the tank inlet opening into the tank and into contact with fluid in the tank.

16. The apparatus according to claim 15, characterized in that the water delivery system comprises a source of water and water control valves movable between open and closed positions to control water flow to the tank.

17. The apparatus according to claim 16, characterized in that the nutrient is a liquid and the nutrient delivery system comprises a nutrient reservoir, a nutrient pump controllable to dispense controlled quantities of the nutrient to the tank.

18. The apparatus according to claim 1, characterized in that the tank includes an air outlet for exit from the tank of excess air delivered to the tank.

19. The apparatus according to claim 18, characterized in that the tank outlet comprises an overflow outlet for flow of fluid from the tank under gravity when fluid in the tank is at a height above a height of the overflow outlet.

20. The apparatus according to claim 19, characterized in that the tank includes a collector to collect fluid flowing from the overflow outlet and deliver the collected fluid to perform the desired utility.

21. An apparatus according to claim 6, wherein the tank has a side wall extending upwardly from the bottom,

the tank outlet is at a height above the bottom,

the impeller rotatable about an axis to discharge fluid impinging on the impeller so as to cause flow of the fluid in the tank which raises fluid in the tank to heights within the tank which increase with increased speed of rotation of the impeller.

22. An apparatus according to claim 21,

wherein the side wall is generally circular in cross-section and disposed generally coaxially about a generally vertically disposed axis about which the impeller is rotatable,

wherein the impeller on rotation creates a standing vortex directing fluid radially outwardly into the side wall and up the side wall.

23. An automated batch process useful for growing bacteria comprising repeating a batch cycle comprising the steps of:

(i) introducing a batch starter population of bacteria, water and nutrients, and growing the bacteria in fluid from the batch starter population to a utility population within a predetermined interval and thereafter,

(ii) repeating a sub-cycle of;

(a) dispensing a dispensed portion of bacteria to perform a desired utility while maintaining a remaining portion of bacteria, and

(b) growing bacteria in the remaining portion to a utility population with the addition of additional water and/or nutrients to the tank,

(iii) followed by, after a number of said sub-cycles, discharging all bacteria from the batch and repeating the batch cycle steps (i) to (iii),

the process being carried out in an apparatus according to claim 1, wherein after each batch cycle and as a step in the next batch cycle the tank used in the previous batch cycle is removed and a tank for the next cycle is coupled in its place such that a batch starter population of bacteria for each cycle is in a tank free of bacteria from a previous batch cycle.

24. An automated batch process useful for growing bacteria comprising repeating a batch cycle comprising the steps of:

(i) introducing a batch starter population of bacteria, water and nutrients, and growing the bacteria in fluid from the batch starter population to a utility population within a predetermined interval and thereafter,

(ii) repeating a sub-cycle of;

(a) dispensing a dispensed portion of bacteria to perform a desired utility while maintaining a remaining portion of bacteria, and

(b) growing bacteria in the remaining portion to a utility population with the addition of additional water and/or nutrients to the tank,

(iii) followed by, after a number of said sub-cycles, discharging all bacteria from the batch and repeating the batch cycle steps (i) to (iii),

the process being carried out in an apparatus according to claim 22, wherein after each batch cycle and as a step in the next batch cycle the tank used in the previous batch cycle is removed and a tank for the next cycle is coupled in its place such that a batch starter population of bacteria for each cycle is in a tank free of bacteria from a previous batch cycle,

wherein during the sub-cycle step (b) of growing bacteria, agitating the fluid by rotating the impeller within a range of speeds which maintain the fluid in the tank at a height below a height of the tank outlet, and

wherein in the sub-cycle step (a), dispensing fluid from the tank by rotating the impeller within a range of speeds which raise the fluid in the tank to a height above the height of the tank outlet.

25. An automated batch process useful for growing bacteria comprising repeating a batch cycle comprising the steps of:

(i) introducing a batch starter population of bacteria, water and nutrients, and growing the bacteria in fluid from the batch starter population to a utility population within a predetermined interval and thereafter,

(ii) repeating a sub-cycle of;

(a) dispensing a dispensed portion of bacteria to perform a desired utility while maintaining a remaining portion of bacteria, and

(b) growing bacteria in the remaining portion to a utility population with the addition of additional water and/or nutrients to the tank,

(iii) followed by, after a number of said sub-cycles, discharging all bacteria from the batch and repeating the batch cycle steps (i) to (iii),

the process being carried out in an apparatus according to claim 22, wherein after each batch cycle and as a step in the next batch cycle the tank used in the previous batch cycle is removed and a tank for the next cycle is coupled in its place such that a batch starter population of bacteria for each cycle is in a tank free of bacteria from a previous batch cycle, including rotating the impeller to maintain a standing wave in the tank during sub-cycle (b) and blowing air downwardly from the top of the tank into the tank into contact with fluid in the standing vortex to provide oxygen from the air to the fluid.

28. A process according to claim 24 including rotating the impeller to maintain a standing wave in the tank during sub-cycle (b) and blowing air downwardly from the top of the tank into the tank into contact with fluid in the standing vortex to provide oxygen from the air to the fluid.

26. A batch process useful for growing bacteria comprising repeating a batch cycle comprising the steps of:

(i) introducing a batch starter population of bacteria and nutrients, and growing the bacteria in fluid from the batch starter population to a utility population within a predetermined interval and thereafter,

(ii) repeating a sub-cycle of;

(a) dispensing a dispensed portion of bacteria while maintaining a remaining portion of bacteria, and

(b) growing bacteria in the remaining portion to a utility population with the addition of additional fluid and/or nutrients to the tank,

the process carried out in an apparatus comprising;

a modular bio-generation tank having a top, a bottom, a side wall extending upwardly from the base, and a tank outlet for flow of fluid from the tank at a height above the bottom,

an agitation mechanism for agitating fluid in the tank comprising an impeller in the tank rotatable about an axis to discharge fluid impinging on the impeller so as to cause flow of the fluid in the tank which raises fluid in the tank to heights within the tank which increase with increased speed of rotation of the impeller,

wherein during the sub-cycle step (b) of growing bacteria, agitating the fluid by rotating the impeller within a range of speeds which maintain the fluid in the tank at a height below a height of the tank outlet, and

wherein in the sub-cycle step (a), dispensing fluid from the tank by rotating the impeller within a range of speeds which raise the fluid in the tank to a height above the height of the tank outlet.

27. A batch process useful for growing bacteria comprising repeating a batch cycle comprising the steps of:

(i) introducing a batch starter population of bacteria and nutrients, and growing the bacteria in fluid from the batch starter population to a utility population within a predetermined interval and thereafter,

(ii) repeating a sub-cycle of;

(a) dispensing a dispensed portion of bacteria while maintaining a remaining portion of bacteria, and

(b) growing bacteria in the remaining portion to a utility population with the addition of additional fluid and/or nutrients to the tank,

the process carried out in an apparatus comprising;

a modular bio-generation tank having a top, a bottom, a side wall extending upwardly from the base, and a tank outlet for flow of fluid from the tank at a height above the bottom,

an agitation mechanism for agitating fluid in the tank comprising;

an impeller which on rotation creates a standing vortex directing fluid radially outwardly into the side wall and up the side wall,

wherein during the sub-cycle step (b) of growing bacteria, agitating the fluid by rotating the impeller within a range of speeds which maintain the fluid in the vortex with the tank at a height below a height of the tank outlet, and blowing air through an opening downwardly into the tank into contact with fluid in the standing vortex to provide oxygen from the air to the fluid.