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Process for making transplantable cardiomyocytes from human embryonic stem cells

  • US 20050054092A1
  • Filed: 03/19/2004
  • Published: 03/10/2005
  • Est. Priority Date: 07/12/2001
  • Status: Active Grant
First Claim
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1. A method of generating a cell composition containing cardiomyocytes or cardiomyocyte precursor cells from primate pluripotent stem (pPS) cells obtained from a human blastocyst, comprising:

  • a) initiating differentiation of the pPS cells in suspension culture by forming embryoid bodies;

    b) culturing the initiated cells so that they differentiate into areas that undergo spontaneous contraction;

    c) harvesting the differentiated cells;

    d) separating the harvested cells into fractions according to their density; and

    e) collecting the cell fractions cells that express cardiac troponin I (cTnI), cardiac troponin T (cTnT), or atrial natriuretic factor (ANF) from an endogenous gene;

    thereby generating a cell composition containing cardiomyocytes or cardiomyocyte precursor cells.

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