Multipotent amniotic fetal stem cells
First Claim
1. A composition comprising multipotent or pluripotent stem cells derived from amniotic fluid.
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Abstract
A source of multipotent amniotic fluid/fetal stem cells (MAFSCs) is disclosed. MAFSC are of fetal origin and have a normal diploid karyotype. These cells are characterized by the following cell surface markers: SSEA3, SSEA4, Tra-1-60, Tra-1-81, Tra-2-54, HLA class I, CD13, CD44, CD49b, CD105 and are distinguished by the absence of the antigen markers CD34, CD45, and HLA Class II, but are distinguished from mouse embryonic stem cells in that these cells do not express the cell surface marker SSEA1. MAFSC express the stem cell transcription factor Oct-4. MAFSC cells can be propagated for an indefinite period of time in continuous culture in an undifferentiated state. The MAFSCs have the ability to differentiate in culture in a regulated manner, into three or more subphenotypes. Cells can then be differentiated into endodermal, mesodermal and ectodermal derived tissues in vitro and in vivo. A method for isolating, identifying, expanding and differentiating MAFSCs is disclosed.
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Citations
37 Claims
- 1. A composition comprising multipotent or pluripotent stem cells derived from amniotic fluid.
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8. A method of preparing multipotent or pluripotent stem cells, comprising:
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harvesting amniotic fluid; and
isolating stem cells from the fluid. - View Dependent Claims (9, 10, 11)
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12. A method of preparing multipotent or pluripotent stem cells, comprising:
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isolating the stem cells from amniotic fluid;
purifying the stem cells; and
growing the stem cells in or on a medium.
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13. A method of preparing undifferentiated multipotent or pluripotent stem cells, comprising:
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isolating said stem cells from amniotic fluid; and
expanding the cells in an undifferentiated state. - View Dependent Claims (16)
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14. A method of preparing differentiated cells, comprising:
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harvesting amniotic fluid;
isolating stem cells from the amniotic fluid;
expanding the isolated stem cells; and
differentiating the stem cells into desired cell types. - View Dependent Claims (15)
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- 17. A multipotent or pluripotent cell derived from amniotic fluid which has been genetically modified.
- 21. A method of promoting bone marrow regeneration in an animal, comprising transplanting MAFSCs or their derivatives into the bone marrow of the animal.
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24. A multipotent stem cell isolated from amniotic fluid, characterized by a) the ability to grow in continuous culture for at least 60 generations, and b) the presence of at least one marker selected from the group consisting of:
- SSEA3, SSEA4, Tra1-60, Tra1-81, Tra2-54, and Oct-4.
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25. A multipotent stem cell isolated from amniotic fluid, characterized by a) the ability to grow in continuous culture for at least 60 generations, and b) the presence of at least two markers selected from the group consisting of:
- SSEA3, SSEA4, Tra1-60, Tra1-81, Tra2-54, and Oct-4.
- View Dependent Claims (26, 27, 28, 29, 30)
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31. A pluripotent stem cell isolated from amniotic fluid, characterized by a) the ability to grow in continuous culture for at least 60 generations, and b) the presence of at least one markers selected from the group consisting of:
- SSEA3, SSEA4, Tra1-60, Tra1-81, Tra2-54, and Oct-4.
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32. A pluripotent stem cell isolated from amniotic fluid, characterized by a) the ability to grow in continuous culture for at least 60 generations, and b) the presence of at least two markers selected from the group consisting of:
- SSEA3, SSEA4, Tra1-60, Tra1-81, Tra2-54, and Oct-4.
- View Dependent Claims (33, 34, 35, 36, 37)
Specification