Grapevine leafroll virus proteins and their uses

• US 20050058989A1
• Filed: 01/21/2004
• Published: 03/17/2005
• Est. Priority Date: 12/21/1995
• Status: Abandoned Application

• Current Assignee: Dennis Gonsalves, Kai-Shu Ling
• Original Assignee: Dennis Gonsalves, Kai-Shu Ling
• Inventors: Gonsalves, Dennis, Ling, Kai-Shu
• Application Number:
  US10/761,983
• Publication Number:
  US 20050058989A1
• Time in Patent Office: Days
• US Class Current: 435/5
• CPC Class Codes: A01H 6/88 Vitaceae, e.g. Vitus [grape]
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 15/8283 for virus resistance
• CPC Class Codes: C12N 2770/00022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 9/127 RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
• CPC Class Codes: C12N 9/90 Isomerases (5.)

Abstract

The invention features antibodies, binding portions thereof, and probes which recognize grapevine leafroll virus proteins and polypeptides.

Claims

1. An antibody or binding portion thereof or probe recognizing a grapevine leafroll virus helicase, RNA-dependent RNA polymerase, hsp70-related, or hsp-90 related protein or polypeptide.

2. The antibody of claim 1, wherein said antibody recognizes a grapevine leafroll virus helicase protein or polypeptide.

3. The antibody of claim 2, wherein said helicase protein or polypeptide has a molecular weight of about 146 to about 151 kDa.

4. The antibody of claim 2, wherein said helicase protein or polypeptide is encoded by a nucleic acid sequence comprising SEQ ID NO:

1.

5. The antibody of claim 2, wherein said helicase protein or polypeptide comprises the amino acid sequence of SEQ ID NO:

2.

6. The antibody of claim 1, wherein said antibody recognizes a grapevine leafroll virus RNA-dependent RNA polymerase protein or polypeptide.

7. The antibody of claim 6, wherein said RNA-dependent RNA polymerase protein or polypeptide has a molecular weight of about 59 to about 63 kDa.

8. The antibody of claim 6, wherein said RNA-dependent RNA polymerase protein or polypeptide is encoded by a nucleic acid sequence comprising SEQ ID NO:

3.

9. The antibody of claim 6, wherein said RNA-dependent RNA polymerase protein or polypeptide comprises the amino acid sequence of SEQ ID NO:

4.

10. The antibody of claim 1, wherein said antibody recognizes a grapevine leafroll virus hsp70-related protein or polypeptide.

11. The antibody of claim 10, wherein said hsp70-related protein or polypeptide has a molecular weight of about 57 to about 61 kDa.

12. The antibody of claim 10, wherein said hsp70-related protein or polypeptide is encoded by a nucleic acid sequence comprising SEQ ID NO:

5.

13. The antibody of claim 10, wherein said hsp70-related protein or polypeptide comprises the amino acid sequence of SEQ ID NO:

6.

14. The antibody of claim 1, wherein said antibody recognizes a grapevine leafroll virus hsp9o-related protein or polypeptide.

15. The antibody of claim 14, wherein said hsp90-related protein or polypeptide has a molecular weight of about 53 to about 57 kDa.

16. The antibody of claim 14, wherein said hsp90-related protein or polypeptide is encoded by a nucleic acid sequence comprising SEQ ID NO:

7.

17. The antibody of claim 14, wherein said hsp90-related protein or polypeptide comprises the amino acid sequence of SEQ ID NO:

8.

18. An antibody that recognizes a protein or polypeptide encoded by the nucleic acid sequence of SEQ ID NO:

11, SEQ ID NO;

13, SEQ ID NO;

15, SEQ ID NO;

17, SEQ ID NO;

19, SEQ ID NO;

21, or SEQ ID NO;

23.

19. The antibody of claim 18, wherein said protein or polypeptide comprises the amino acid sequence of SEQ ID NO:

12.

20. The antibody of claim 18, wherein said protein or polypeptide comprises the amino acid sequence of SEQ ID NO:

14.

21. The antibody of claim 18, wherein said protein or polypeptide comprises the amino acid sequence of SEQ ID NO:

16.

22. The antibody of claim 18, wherein said protein or polypeptide comprises the amino acid sequence of SEQ ID NO:

18.

23. The antibody of claim 18, wherein said protein or polypeptide comprises the amino acid sequence of SEQ ID NO:

20.

24. The antibody of claim 18, wherein said protein or polypeptide comprises the amino acid sequence of SEQ ID NO:

22.

25. The antibody of claim 18, wherein said protein or polypeptide comprises the amino acid sequence of SEQ ID NO:

24.