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Direct SNP detection with unamplified DNA

  • US 20050059030A1
  • Filed: 12/12/2003
  • Published: 03/17/2005
  • Est. Priority Date: 12/12/2002
  • Status: Abandoned Application
First Claim
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1. A method for detecting a target nucleic acid sequence in a sample, the sample comprising nucleic acid molecules of higher biological complexity relative to amplified nucleic acid molecules and the target nucleic acid sequence differs from one or more nucleic acid sequences by at least one nucleotide, the method comprising the steps of:

  • a) providing an addressable substrate having a capture oligonucleotide bound thereto, wherein the capture oligonucleotide has a sequence that is complementary to at least part of a first portion of the target nucleic acid sequence;

    b) providing a detection probe comprising detector oligonucleotides, wherein the detector oligonucleotides have a sequence that is complementary to at least part of a second portion of the target nucleic acid sequence of step (a);

    c) contacting the sample with the substrate and the detection probe under conditions that are effective for the hybridization of the capture oligonucleotide to the first portion of the target nucleic acid sequence and the hybridization of the detection probe to the second portion of the target nucleic acid sequence and to allow for discrimination between the target and said one or more nucleic acid sequences that differ by at least one nucleotide; and

    d) detecting whether the capture oligonucleotide and detection probe hybridized with the first and second portions of the target nucleic acid sequence.

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