Nucleotide derivative and DNA microarray
First Claim
1. A nucleotide derivative having a fluorescent dye intercalator bound via a linker to a pyrimidine base or purine base, which is in case of existing as a member of a single-stranded sequence:
- a cytosine derivative (2) of which fluorescent dye emits light most intensely when the confronting base is guanine;
an adenine derivative (3) of which fluorescent dye emits light most intensely when the confronting base is cytosine;
a guanine derivative (4) of which fluorescent dye emits light most intensely when the confronting base is cytosine or thymine/uracil; and
an adenine derivative (5) of which fluorescent dye emits light most intensely when the confronting base is thymine/uracil.
1 Assignment
0 Petitions
Accused Products
Abstract
A novel nucleotide derivative, in case of existing as a member of a single-stranded sequence, undergoing a change in the fluorescent signal intensity depending on the corresponding base type in the partner strand with which the single-stranded sequence is hybridized, and which is a thymine/uracil derivative (1) emitting light most intensely when a confronting base in the partner strand with which the single-stranded nucleotide sequence is hybridized is adenine; a cytosine derivative (2) emitting light most intensely when the confronting base is guanine; an adenine derivative (3) emitting light most intensely when the confronting base is cytosine; a guanine derivative (4) emitting light most intensely when the confronting base is cytosine or thymine/uracil; and an adenine derivative (5) emitting light most intensely when the confronting base is thymine/uracil.
4 Citations
31 Claims
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1. A nucleotide derivative having a fluorescent dye intercalator bound via a linker to a pyrimidine base or purine base, which is in case of existing as a member of a single-stranded sequence:
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a cytosine derivative (2) of which fluorescent dye emits light most intensely when the confronting base is guanine;
an adenine derivative (3) of which fluorescent dye emits light most intensely when the confronting base is cytosine;
a guanine derivative (4) of which fluorescent dye emits light most intensely when the confronting base is cytosine or thymine/uracil; and
an adenine derivative (5) of which fluorescent dye emits light most intensely when the confronting base is thymine/uracil. - View Dependent Claims (2, 3, 4, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 28, 29, 30, 31)
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5-8. -8. (Canceled)
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16. A method for determining a single base type X in the partner strand with which the single-stranded nucleotide sequence of the third invention is hybridized, wherein the base type X is determined as:
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(i) adenine when the fluorescent dye of the thymine/uracil derivative (1) in the single-stranded nucleotide sequence emits light most intensely;
(ii) guanine when the fluorescent dye of the cytosine derivative (2) in the sequence emits light most intensely;
(iii) cytosine when the fluorescent dye of the adenine derivative (3) in the sequence emits light most intensely; and
,(iv) thymine/uracil when the fluorescent dye of the adenine derivative (5) in the sequence emits light most intensely.
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27. A method for assessing an amount of target nucleotide sequences, which comprises the steps of hybridizing the single-stranded nucleotide sequence of the first or the fifth invention with target nucleotide sequences of which sequences information are known, and measuring fluorescent intensity of the nucleotide derivative.
Specification