Impedimetric biosensor and its use for rapid detection of bacterial pathogens in solution

• US 20050059105A1
• Filed: 07/21/2004
• Published: 03/17/2005
• Est. Priority Date: 07/25/2003
• Status: Abandoned Application

• Current Assignee: The Board of Trustees of Michigan State University (Michigan State University)
• Original Assignee: The Board of Trustees of Michigan State University (Michigan State University)
• Inventors: Radke, Stephen, Alocilja, Evangelyn C.
• Application Number:
  US10/895,664
• Publication Number:
  US 20050059105A1
• Time in Patent Office: Days
• US Class Current: 435/7.320
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/569 for microorganisms, e.g. protozoa, bacteria, viruses
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

A method and device for the rapid detection of microorganisms by detection of a change of impedance as a result of the microorganism being bound between interdigitated electrodes is described. In particular, the present invention relates to a handheld device to be used for the detection and, in many instances, the identification of the microorganism.

Claims

1. A device for detecting a microorganism which comprises:

(a) a holder means with an electrode means;

(b) a detection sensor mounted on the holder means in contact with the electrical means, the sensor having spaced apart interdigitated electrodes providing a capacitor and with a capture reagent for the microorganism at least between the electrodes, wherein widths of a space between the electrodes and of the electrodes are each about 5 microns or less, and wherein when the microorganism in a liquid is captured by the capture reagent, the detection sensor is interrogated with an electric circuit producing a change of impedance from the detection sensor without the bound microorganism, thereby detecting the presence of the microorganism.

2. The device of claim 1 wherein the electrode means are connected to a circuit for measuring the impedance.

3. The device of claim 2 wherein the device is adapted to be removed from the liquid after the microorganisms are bound to the detection sensor and then to be connected to the circuit for the interrogation of the sensor.

4. The device of any one of claims 1, 2 or wherein with a reference sensor is provided, adjacent 3 the detection sensor, without the capture reagent for detecting the change of impedance.

5. The device of any one of claims 1, 2 or 3 wherein the electrodes have a 4 μ

m (4000 nm) spacing and a 3 μ

m (3000 nm) width, and are provided on a non-conductive substrate.

6. The device of any one of claims 1, 2 or 3 wherein the captive reagent is specific for at least a genus classification of the microorganism and the microorganism is identified at least as to the genus classification.

7. A method for detecting a microorganism which comprises:

(a) providing a device which comprises a holder means with an electrode means;

a detection sensor mounted on the holder means in contact with the electrode means, the sensor having spaced apart interdigitated electrodes providing a capacitor and with a capture reagent for the microorganism at least between the electrodes and wherein widths of spaces between each of the electrodes and of the electrodes are each about 5 microns or less, and wherein when the microorganism in a liquid is captured by the capture reagent, the detection sensor is interrogated with an electric circuit producing a change of impedance from the detection sensor without the bound microorganism, thereby detecting the presence of the microorganism;

(b) inserting the detection sensor into a liquid suspected of containing the microorganism;

(c) washing the biosensor to remove any unbound material; and

(d) determining the change of impedance from the detection sensor by interrogating the detection sensor with the electronic circuit to thereby detect the microorganisms.

8. The method of claim 7 wherein the captive reagent is specific for at least a genus classification of the organism and the microorganism is identified at least as to the genus classification.

9. The method of claims 7 or 8 wherein a reference sensor is provided, adjacent to the detection sensor, without the capture reagent for detecting the change of impedance in the presence of the microorganism.

10. A device for detecting an analyte in a solution, which comprises:

a biosensor comprising

(i) a substrate material having a surface;

(ii) an interdigitated array of at least two electrodes disposed on the surface of the substrate wherein a width of a space between the electrodes and of the electrodes are each about 5 microns or less; and

(iii) at least one covalently bound antibody which is specific for the analyte immobilized at least on the surface of the substrate in the space between the electrodes.

11. The device of claim 10 wherein the substrate material is a planar semiconductor device.

12. The device of claim 11 wherein the substrate material is a silicon wafer with an insulator layer passivated thereon.

13. The device of claim 12 wherein the insulator layer is silicon oxide.

14. The device of claim 10 wherein the interdigitated electrodes comprise a metal selected from the group consisting of platinum, palladium, gold, indium tin oxide, iridium, rhodium, osmium, copper, silver, and mixtures thereof.

15. The device of claim 10 wherein a size of the surface between the interdigitated electrodes corresponds to a size range of at least one of the covalently bound antibodies.

16. The device of claim 15 wherein the size is about 4 μ

m (4000 nm).

17. The device of claim 10 wherein the antibody is immobilized on the surface via a heterobifunctional crosslinker or amine crosslinker.

18. The device of claim 10 wherein the heterobifunctional crosslinker is an N-γ

-maleimidobutyryloxy succinimide ester.

19. The device of claim 10 wherein the antibodies are recombinant antibodies.

20. The device of claim 19 wherein the recombinant antibodies are selected from the group consisting of scFV polypeptides and V_H chain polypeptides.

21. A method for detecting an analyte in a liquid sample, which comprises:

(a) providing a device comprising a biosensor comprising

(i) a substrate material having a surface;

(ii) an interdigitated array of at least two electrodes disposed on the surface of the substrate wherein widths of a space between the electrodes and of the electrodes are each about 5 microns or less in width; and

(iii) at least one covalently bound antibody which is specific for the analyte immobilized at least on the surface of the substrate in the space between the electrodes; and

(b) immersing the biosensor of the device in the liquid sample for a time sufficient for the antibody to bind the analyte;

(c) removing the biosensor of the device from the liquid sample and immersing the biosensor of the device in a neutral solution; and

(d) applying a current to the biosensor of the device and measuring the impedance of the current wherein an increase in the impedance indicates the liquid sample has the analyte.

22. The method of claim 21 wherein the substrate material is a planar semiconductor device.

23. The method of claim 22 wherein the substrate material is a silicon wafer with an insulator layer passivated thereon.

24. The method of claim 23 wherein the insulator layer is silicon oxide.

25. The method of claim 21 wherein the interdigitated electrodes comprises a metal selected from the group consisting of platinum, palladium, gold, indium tin oxide, iridium, rhodium, osmium, copper, silver, and mixtures thereof.

26. The method of claim 21 wherein a size of the surface between the interdigitated electrodes corresponds to a size range of at least one of the covalently bound antibodies.

27. The method of claim 26 wherein the size is about 4 μ

m (4000 nm).

28. The method of claim 21 wherein the antibody is immobilized on the surface via a heterobifunctional crosslinker or a homobifunctional crosslinker.

29. The method of claim 21 wherein the heterobifunctional crosslinker is an N-γ

-maleimidobutyryloxy succinimide ester.

30. The method of claim 21 wherein the analyte is selected from the group consisting of bacteria, parasites, viruses, toxins, and enzymes.

31. The method of claim 21 wherein the antibodies are recombinant antibodies.

32. The method of claim 31 wherein the recombinant antibodies are selected from the group consisting of scFV polypeptides and V_H chain polypeptides.

33. A method for detecting a microorganism in a liquid sample, which comprises:

(a) providing a device comprising a biosensor comprising

(i) a substrate material having a surface;

(ii) an interdigitated array of at least two electrodes disposed on the surface of the substrate wherein widths of a space between the electrodes and of the electrodes are each about 5 microns or less in width;

(iii) at least one covalently bound antibody which is specific for the analyte immobilized at least on the surface of the substrate in the space between the electrodes; and

(b) immersing the biosensor of the device in the liquid sample for a time sufficient for the antibody to bind the microorganism;

(c) removing the biosensor of the device from the liquid sample and immersing the biosensor of the device in a neutral solution; and

(d) applying a current to the biosensor of the device and measuring the impedance of the current wherein an increase in the impedance indicates the liquid sample has the microorganism.

35. The method of claim 33 or 34 wherein the substrate material is a planar semiconductor device.

36. The method of claim 35 wherein the substrate material is a silicon wafer with an insulator layer passivated thereon.

37. The method of claim 36 wherein the insulator layer is silicon oxide.

38. The method of claim 33 or 34 wherein the interdigitated electrodes comprises a metal selected from the group consisting of platinum, palladium, gold, indium tin oxide, iridium, rhodium, osmium, copper, silver, and mixtures thereof.

39. The method of claim 33 or 34 wherein a size of the surface between the interdigitated electrodes corresponds to a size range of at least one of the covalently bound antibodies.

40. The method of claim 39 wherein the size is about 4 μ

m (4000 nm).

41. The method of claim 33 or 34 wherein the antibody is immobilized on the surface via a heterobifunctional crosslinker or a homobifunctional crosslinker.

42. The method of claim 33 or 34 wherein the heterobifunctional crosslinker is an N-γ

-maleimidobutyryloxy succinimide ester.

43. The method of claim 33 or 34 wherein the support means is an elongated probe.

44. The method of claim 33 or 34 wherein the microorganism is a bacterium.

45. The method of claim 44 wherein the bacterium is Escherichia coli strain O157:

H7.

46. The method of claim 33 or 34 wherein the antibodies are recombinant antibodies.

47. The method of claim 46 wherein the recombinant antibodies are selected from the group consisting of scFV polypeptides and V_H chain polypeptides.

34. A method for identifying a microorganism in a liquid sample, which comprises:

(a) providing a device comprising a biosensor comprising

(i) a substrate material having a surface;

(ii) an interdigitated array of at least two electrodes disposed on the surface of the substrate wherein widths of a space between the electrodes and of the electrodes are each about 5 microns or less; and

(iii) at least one covalently bound antibody which is specific for the analyte immobilized at least on the surface of the substrate in the space between the electrodes; and

(b) immersing the biosensor of the device in the liquid sample for a time sufficient for the antibody to bind the microorganism;

(c) removing the biosensor of the device from the liquid sample and immersing the biosensor of the device in water; and

(d) applying a current to the biosensor of the device and measuring the impedance of the current wherein an increase in the impedance to a particular amount identifies the microorganism.