Method for obtaining a fluid sample

• US 20050059161A1
• Filed: 10/26/2004
• Published: 03/17/2005
• Est. Priority Date: 05/14/1999
• Status: Active Grant

• Current Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Original Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Inventors: Kacian, Daniel L., Carter, Nick M., Anderson, Bruce W., Iheme, Mordi I.
• Granted Patent: US 7,648,680 B2
• Time in Patent Office: Days
• US Class Current: 436/174
• CPC Class Codes: B01L 2200/026 Fluid interfacing between devices or objects, e.g. connectors, inlet details
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/141 Preventing contamination, tampering
• CPC Class Codes: B01L 2300/042 Caps; Plugs
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 2300/047 Additional chamber, reservoir
• CPC Class Codes: B01L 2300/048 enabling gas exchange, e.g. vents
• CPC Class Codes: B01L 2300/0609 Holders integrated in container to position an object
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 3/0275 Interchangeable or disposable dispensing tips
• CPC Class Codes: B01L 3/5029 using swabs
• CPC Class Codes: B01L 3/50825 Closing or opening means, corks, bungs closures for containers B65D; means for removing stoppers B67B7/02
• CPC Class Codes: G01N 35/1079 with means for piercing stoppers or septums
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/25125 Digestion or removing interfering materials
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

Method for obtaining a fluid sample from a collection device which includes assembling cap and fluid-holding vessel components of the collection device to contain and isolate a specimen retrieval device adjacent a side wall of the vessel. The position of the specimen retrieval device within the collection device is such that the specimen retrieval device does not substantially interfere with the movement of a fluid transfer device into or out of the assembled collection device. The fluid transfer device is used to draw and remove a fluid substance from the collection device for analysis.

Claims

1. A method for obtaining a fluid sample comprising:

a) assembling a collection device comprising a cap and a fluid-holding vessel to contain and isolate a specimen retrieval device adjacent a side wall of the vessel;

b) entering the assembled collection device of step a) with a fluid transfer device such that the specimen retrieval device does not substantially interfere with the movement of the fluid transfer device into or out of the collection device;

c) drawing at least a portion of a fluid substance present in the collection device into the fluid transfer device; and

d) removing the fluid transfer device from the collection device.

2. The method of claim 1, wherein the cap and vessel include mated threads for fixedly associating the cap and vessel.

3. The method of claim 2, wherein the assembled collection device of step a) is substantially leak-proof.

4. The collection device of claim 1, wherein the cap and vessel are each formed of an injection molded plastic.

5. The method of claim 1, wherein the specimen retrieval device is a swab.

6. The method of claim 5, wherein the swab includes a score line to facilitate snapping-off an upper portion of the swab above the score line.

7. The method of claim 6 further comprising the steps of snapping-off and removing the upper portion of the swab.

8. The method of claim 1, wherein the fluid transfer device is a plastic pipette tip.

9. The method of claim 8, wherein the penetrating step requires applying a force of less than about 8 pounds force to a surface of the cap.

10. The method of claim 1, wherein the cap includes a plurality radially extending striations.

11. The method of claim 1, wherein the fluid substance is suspected of containing a target nucleic acid.

12. The method of claim 11 further comprising the step of amplifying a target nucleic acid sequence contained in the target nucleic acid.

13. The method of claim 12, wherein the amplifying step is performed using a polymerase chain reaction procedure.

14. The method of claim 12, wherein the amplifying step is performed using a transcription-mediated amplification procedure.

15. The method of claim 12 further comprising the step of detecting the presence of the target nucleic acid in the fluid substance.

16. The method of claim 11 further comprising the step of detecting the presence of the target nucleic acid in the fluid substance.

17. The method of claim 1 further comprising the step of inhibiting the release of an aerosol or bubbles from the collection device.

18. The method of claim 17, wherein the inhibiting step is carried out using a wick contained within the cap or an overcap.

19. The method of claim 18, wherein the wick is a pile fabric.

20. The method of claim 12 further comprising the step of inhibiting the release of an aerosol or bubbles from the collection device.

21. The method of claim 20, wherein the inhibiting step is carried out using a wick contained within the cap or an overcap.

22. The method of claim 21, wherein the cap or overcap includes a seal for retaining the wick within the cap or overcap.

23. The method of claim 22, wherein the wick is a pile fabric.