Computer software and algorithms for systems biologically linked to cellular phenotype
First Claim
1. An automated method for identifying agents that cause a phenotypic change in a cell comprising the steps of:
- providing receptacles in an array;
providing a statistical design including generic factor names, factor levels, and experimental runs;
utilizing a software program to generate a computer representation of said statistical design, said computer representation being generated by automatically mapping the identities of agents to said generic factor names, by mapping the concentration or amounts of said agents to said factor levels, and by mapping the locations of said receptacles within said array to said experimental runs;
placing different mixtures of single said agents into select ones of said receptacles in said array according to said computer representation of said statistical design;
contacting said placed mixtures with said cells;
acquiring data indicative of a phenotypic change in said contacted cells;
utilizing a processor including an algorithm for comparing said phenotypic data with said statistical design to identify which of said mixtures of single agents and/or or which of said single agents in said mixtures are effective in causing said phenotypic change in said contacted cells; and
storing said statistical design, said agent identities, said computer representation of said statistical design, said acquired data, and the results of said algorithm comparison in one or more databases.
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Accused Products
Abstract
An automated method and system for identifying agents capable of eliciting a phenotypic change in a cell-type is provided. The method includes the steps of: providing receptacles in an array; and providing a statistical design including generic factor names, factor levels and experimental runs. The method further includes the steps of utilizing a software program to generate a computer representation of the statistical design by automatically mapping the identities of the agents to the generic factor names, by mapping the concentration or amounts of the agents to the factor levels and by mapping the locations of the receptacles within the array to the experimental runs. The method also includes placing different mixtures of single agents into receptacles in the array based on the computer representation of the statistical design; contacting the placed mixtures with cells; acquiring experimental data from the contacted cells; and utilizing a processor including an algorithm for comparing the acquired data with the statistical design to identify particular agent mixtures or single agents that cause a phenotypic change in the contacted cells.
40 Citations
36 Claims
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1. An automated method for identifying agents that cause a phenotypic change in a cell comprising the steps of:
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providing receptacles in an array;
providing a statistical design including generic factor names, factor levels, and experimental runs;
utilizing a software program to generate a computer representation of said statistical design, said computer representation being generated by automatically mapping the identities of agents to said generic factor names, by mapping the concentration or amounts of said agents to said factor levels, and by mapping the locations of said receptacles within said array to said experimental runs;
placing different mixtures of single said agents into select ones of said receptacles in said array according to said computer representation of said statistical design;
contacting said placed mixtures with said cells;
acquiring data indicative of a phenotypic change in said contacted cells;
utilizing a processor including an algorithm for comparing said phenotypic data with said statistical design to identify which of said mixtures of single agents and/or or which of said single agents in said mixtures are effective in causing said phenotypic change in said contacted cells; and
storing said statistical design, said agent identities, said computer representation of said statistical design, said acquired data, and the results of said algorithm comparison in one or more databases. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 36)
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32. A system for identifying agents that cause a phenotypic change in a cell, comprising:
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an array of receptacles, selective ones of which are for receiving (i) different mixtures of single said agents, and (ii) fluid including said cells;
a statistical design including generic factor names, factor levels, and experimental runs;
a software program for generating a computer representation of said statistical design, wherein said software program automatically maps the identities of said agents to said generic factor names, maps the concentration of or amounts of said agents to said factor levels, and maps the locations of said receptacles in said array to said experimental runs;
acquired experimental data indicative of said phenotypic change in said cells;
a processor including an algorithm for comparing said experimental data with said statistical design to identify which of said mixtures of single agents and/or which of said single agents in said mixtures are effective in causing said phenotypic change in said cells; and
one or more databases for storing said statistical design, said agent identities, said computer representation of said statistical design, said acquired experimental data, and the results of said algorithm comparison. - View Dependent Claims (33, 34, 35)
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Specification