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Expression of dominant negative transmembrane receptors in the milk of transgenic animals

  • US 20050060766A1
  • Filed: 09/15/2004
  • Published: 03/17/2005
  • Est. Priority Date: 09/15/2003
  • Status: Abandoned Application
First Claim
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1. A method for cloning a non-human mammal through a nuclear transfer process comprising:

  • (i) obtaining desired differentiated mammalian cells to be used as a source of donor nuclei;

    (ii) obtaining at least one oocyte from a mammal of the same species as the cells which are the source of donor nuclei;

    (iii) enucleating said at least one oocyte;

    (iv) transferring the desired differentiated cell or cell nucleus into the enucleated oocyte;

    (v) simultaneously fusing and activating the cell couplet to form a transgenic embryo;

    (vii) culturing said transgenic embryo(es) until greater than the 2-cell developmental stage; and

    (viii) transferring said transgenic embryo into a host mammal such that the embryo develops into a fetus;

    wherein the desired differentiated cell or cell nucleus contains a recombinant transgene; and

    , wherein said recombinant transgene encodes a recombinant transmembrane receptor protein of interest.

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