Mass spectrometer system
First Claim
1. A mass spectroscopy system comprising:
- ionization means for ionizing a substance as an object of measurement for a mass spectrometer; and
means for selecting an ion species with a specific mass-to-charge ratio m/z from ions produced by said ionization means and dissociating the same, wherein the selection, dissociation and measurement of the ion species as the measurement object are repeated in a plurality of stages, said mass spectroscopy system further comprising;
mass spectroscopy data acquisition means for performing the selection and dissociation of an ion species n−
1 times (n≧
1, where n is an integer) and acquiring a peak of measurement intensity against the mass-to-charge ratio of the ion that has been selected and dissociated;
correspondence determination means for comparing the peak of measurement intensity against the mass-to-charge ratio of the ion that is obtained by the mass spectroscopy data acquisition means with the characteristics data of a certain ion species in order to determine the possibility of correspondence of the ion that has been selected and dissociated to the certain ion species; and
next-analysis content determination means for determining the analysis content in an n-th stage mass spectroscopy based on the result of determination by said correspondence determination means.
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Accused Products
Abstract
During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided. The information contained in the MSn spectrum is effectively utilized in each step of the MSn involving a multi-stage dissociation and mass spectroscopy (MSn), so that the flows for the determination of the next analysis content and the selection of the parent ion for the MSn+1 analysis, for example, can be optimized within the real time of measurement and with high efficiency and accuracy. Thus, a target of concern to the user can be subjected to tandem mass spectroscopy without wasteful measurement.
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Citations
31 Claims
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1. A mass spectroscopy system comprising:
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ionization means for ionizing a substance as an object of measurement for a mass spectrometer; and
means for selecting an ion species with a specific mass-to-charge ratio m/z from ions produced by said ionization means and dissociating the same, wherein the selection, dissociation and measurement of the ion species as the measurement object are repeated in a plurality of stages, said mass spectroscopy system further comprising;
mass spectroscopy data acquisition means for performing the selection and dissociation of an ion species n−
1 times (n≧
1, where n is an integer) and acquiring a peak of measurement intensity against the mass-to-charge ratio of the ion that has been selected and dissociated;
correspondence determination means for comparing the peak of measurement intensity against the mass-to-charge ratio of the ion that is obtained by the mass spectroscopy data acquisition means with the characteristics data of a certain ion species in order to determine the possibility of correspondence of the ion that has been selected and dissociated to the certain ion species; and
next-analysis content determination means for determining the analysis content in an n-th stage mass spectroscopy based on the result of determination by said correspondence determination means. - View Dependent Claims (2, 3, 4, 5, 7, 26, 27, 28, 29, 30, 31)
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6. A mass spectroscopy system comprising:
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ionization means for ionizing a substance as an object of measurement for a mass spectrometer; and
mass spectroscopy means for selecting an ion species with a specific mass-to-charge ratio m/z from ions produced by said ionization means and dissociating the same, and for repeating the selection, dissociation and measurement of the ion species as the measurement object in a plurality of stages, said mass spectroscopy system further comprising;
mass spectroscopy data acquisition means for acquiring information about a peak of measurement intensity against the mass-to-charge ratio of an n-th stage ion obtained by said mass spectroscopy means;
isotope peak determination means for determining an isotope peak based on ion mass spectroscopy data acquired by said mass spectroscopy data acquisition means; and
next analysis content determination means for determining the next n-th stage analysis content based on the isotope peak determined by said isotope peak determination means.
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8. A mass spectroscopy system comprising:
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an ion source for ionizing a sample;
a mass spectroscopy portion for performing a primary mass spectroscopy on an ion obtained by ionizing said sample and performing a secondary mass spectroscopy, following said primary mass spectroscopy, on a dissociated ion produced by dissociating said ion;
an RF power supply for applying an RF voltage for eliminating ions that are not analysis objects prior to said primary mass spectroscopy; and
a control portion for outputting a instruction for the elimination of the non-analysis object ions to said RF power supply. - View Dependent Claims (9, 10, 11, 12, 13)
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14. A mass spectrum measurement employing an apparatus comprising an ion source, a mass spectroscopy portion, and an RF power supply, said method comprising:
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ionizing a sample using said ion source;
selectively eliminating non-analysis object ions from the ions obtained by the ionization, using said RF power supply;
performing a primary mass spectroscopy on said ion in said mass spectroscopy portion, following the elimination step; and
performing a secondary mass spectroscopy on a dissociated ion produced by dissociating said ion following said primary mass spectroscopy. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22)
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23. A mass spectroscopy apparatus comprising:
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an ion source for ionizing a sample;
a first database in which a data sequence of an analysis object candidate substance is recorded;
a control portion for issuing an instruction for selecting an analysis object ion that corresponds to the data in said first database; and
a mass spectroscopy portion for performing a primary mass spectroscopy on an ion obtained by ionizing said sample and subjecting a dissociated ion produced by the dissociation of the selected analysis object ion to a secondary mass spectroscopy. - View Dependent Claims (24, 25)
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Specification