Biosensor and method of manufacturing biosensor

• US 20050063870A1
• Filed: 08/05/2004
• Published: 03/24/2005
• Est. Priority Date: 09/01/2003
• Status: Active Grant

• Current Assignee: Seiko Epson Corporation (Seiko Group)
• Original Assignee: Seiko Epson Corporation (Seiko Group)
• Inventors: Kondo, Takayuki, Fukushima, Hitoshi
• Granted Patent: US 7,407,628 B2
• Time in Patent Office: Days
• US Class Current: 422/82.50
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6454 using an integrated detector array

Abstract

Aspects of the invention can be an inexpensive biosensor capable of measuring a number of samples in a short time, and suitable for only one-time use (expendable). The biosensor according to the invention can include a light transmissive substrate, a probe fixing region provided on one face of the light transmissive substrate, a light emitting element provided on the other face of the substrate that irradiates the probe fixing region from the back side thereof, and a light receiving element provided on the other face of the substrate that detects the light intensity of the reflected light from the back side of the probe fixing region. Thereby, a biosensor formed in one substrate can be obtained.

Claims

1. A biosensor, comprising:

a light transmissive substrate;

a probe fixing region provided on a first face of the light transmissive substrate; and

a light emitting element provided on a second face of the light transmissive substrate that irradiates the probe fixing region from a back side thereof.

5. The biosensor according to claim 1, the light transmissive substrate being formed by bonding a first substrate in which the probe fixing region is formed and a second substrate in which at least either one of the light emitting element and the light receiving element is formed.

7. The biosensor according to claim 1, the probe fixing region in the light transmissive substrate being set on a microlens or a microprism formed in the light transmissive substrate.

8. The biosensor according to claim 1, the light emitting element or the light receiving element being formed by a thin film circuit.

9. The biosensor according to claim 1, the light emitting element including at least any one of a light emitting diode, vertical-cavity surface-emitting laser, and an organic EL element.

11. The biosensor according to claim 1, a specific biological sample and a uniquely acting probe being fixed to the probe fixing region.

12. The biosensor according to claim 11, the biological sample including at least any one of DNA, proteins, and antibodies.

13. The biosensor according to claim 1, a lyophilic processing being carried out to the probe fixing region in the light transmissive substrate, and the lyophobic processing being carried out to the other regions.

14. The biosensor according to claim 1, a metal thin film being formed on the probe fixing region in the light transmissive substrate.

2. A biosensor, comprising:

a light transmissive substrate;

a probe fixing region provided on a first face of the light transmissive substrate; and

a light receiving element provided on a second face of the light transmissive substrate that detects light intensity on a light transmissive substrate side of the probe fixing region.

6. The biosensor according to claim 2, the light transmissive substrate including depositing a first substrate in which the probe fixing region is formed and a second substrate in which the light receiving element is formed, through a filter layer that transmits light having a specific wavelength.

10. The biosensor according to claim 2, the light receiving element including at least any one of a photo-diode, a photo-transistor, and CCD.

3. A biosensor, comprising:

a light transmissive substrate;

a probe fixing region provided on a first face of the light transmissive substrate;

a light emitting element provided on a second face of the light transmissive substrate that irradiates the probe fixing region from the light transmissive substrate side; and

a light receiving element provided on the second face of the light transmissive substrate that detects light intensity of the reflected light from a light transmissive substrate side of the probe fixing region.

4. A biosensor, comprising:

a light transmissive substrate;

a plurality of probe fixing regions provided on a first face of the light transmissive substrate;

a light shielding region formed in the light transmissive substrate that shields the light between the probe fixing regions; and

a light emitting element that irradiates the probe fixing region from a light transmissive substrate side, and a light receiving element that detects light intensity of the reflected light from the light transmissive substrate side of the probe fixing region, the light emitting element and the light receiving element being provided in each probe fixing region on a second face of the light transmissive substrate.

15. A biosensor constituted by sequentially arranging in one direction:

a substrate, in which a thin film light emitting element is formed on one face;

a light transmissive substrate, in which a probe fixing region is formed on one face;

a substrate, in which a thin film filter that transmits light having a specific wavelength is formed; and

a substrate, in which a thin film light receiving element is formed on one face.

16. A biosensor constituted by sequentially arranging in one direction:

a substrate, in which a plurality of thin film light emitting elements are arranged on one face;

a light transmissive substrate, in which a plurality of probe fixing regions are arranged corresponding to the plurality of thin film light emitting elements on one face;

a substrate, in which a thin film filter that transmits light having a specific wavelength is formed; and

a substrate, in which a plurality of thin film light receiving elements are arranged corresponding to the plurality of probe fixing regions on one face.

17. A method of manufacturing a biosensor, comprising:

forming a probe fixing region on a part of or a whole of a first face of a light transmissive substrate by carrying out a lyophilic processing or forming a metal thin film; and

transferring at least either one of a light emitting element chip and a light receiving element chip to a second face of the substrate, corresponding to the probe fixing region.

19. The method of manufacturing a biosensor according to claim 17, further comprising forming a probe in the probe fixing region of the substrate.

18. A method of manufacturing a biosensor, comprising:

forming a probe fixing region on one face of a light transmissive first substrate;

transferring at least either one of a light emitting element chip and a light receiving element chip on one face of a second substrate, corresponding to the probe fixing region; and

bonding the first and the second substrate.