Continuous and non-continuous flow bioreactor
First Claim
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1. A method of amplifying at least one RNA, the method comprising:
- flowing one or more RNA amplification reagent into a microscale chamber; and
, amplifying one or more template nucleic acid to produce one or more RNA amplicon in the microscale reaction chamber under conditions of constant or semi-constant flow, wherein one or more reaction parameter is optimized to provide production of the RNA amplicons in the reaction chamber, or flow of the amplicons out of the chamber, or both.
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Abstract
Methods and systems for performing continuous amplification of RNA and other nucleic acids are provided. Expression profiling using the continuous flow RNA amplification systems are also provided.
48 Citations
27 Claims
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1. A method of amplifying at least one RNA, the method comprising:
flowing one or more RNA amplification reagent into a microscale chamber; and
, amplifying one or more template nucleic acid to produce one or more RNA amplicon in the microscale reaction chamber under conditions of constant or semi-constant flow, wherein one or more reaction parameter is optimized to provide production of the RNA amplicons in the reaction chamber, or flow of the amplicons out of the chamber, or both.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A method of detecting presence or absence of one or more target RNA in a biological sample, the method comprising:
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performing one of more reverse transcription reaction on sample RNA from the biological sample to produce one or more cDNA;
flowing transcription reagents into contact with the one or more cDNA;
performing one or more expression reaction on the cDNA under conditions of continuous or semi-continuous flow; and
,detecting one or more RNA amplicon, or lack thereof, produced by the expression reaction, thereby detecting presence or absence of the target RNA.
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Specification